EC 5.1.3.23 - UDP-2,3-diacetamido-2,3-dideoxyglucuronic acid 2-epimerase

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IntEnz Enzyme Nomenclature
EC 5.1.3.23

Names

Accepted name:
UDP-2,3-diacetamido-2,3-dideoxyglucuronic acid 2-epimerase
Other names:
UDP-GlcNAc3NAcA 2-epimerase
UDP-α-D-GlcNAc3NAcA 2-epimerase
2,3-diacetamido-2,3-dideoxy-α-D-glucuronic acid 2-epimerase
WbpI
WlbD
Systematic name:
2,3-diacetamido-2,3-dideoxy-α-D-glucuronate 2-epimerase

Reaction

Comments:

This enzyme participates in the biosynthetic pathway for UDP-α-D-ManNAc3NAcA (UDP-2,3-diacetamido-2,3-dideoxy-α-D-mannuronic acid), an important precursor of the B-band lipopolysaccharide of Pseudomonas aeroginosa serotype O5 and of the band-A trisaccharide of Bordetella pertussis, both important respiratory pathogens [1]. The enzyme is highly specific as UDP-α-D-GlcNAc, UDP-α-D-GlcNAcA (UDP-2-acetamido-2-deoxy-α-D-glucuronic acid) and UDP-α-D-GlcNAc3NAc (UDP-2,3-diacetamido-2,3-dideoxy-α-D-glucose) cannot act as substrates [1].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot:

References

  1. Westman, E.L., McNally, D.J., Rejzek, M., Miller, W.L., Sri Kannathasan, V., Preston, A., Maskell, D.J., Field, R.A., Brisson, J.-R. and Lam, J.S.
    Identification and biochemical characterization of two novel UDP-2,3-diacetamido-2,3-dideoxy-α-D-glucuronic acid 2-epimerases from respiratory pathogens.
    Biochem. J. 405: 123-130 (2007). [PMID: 17346239]
  2. Westman, E.L., McNally, D.J., Rejzek, M., Miller, W.L., Sri Kannathasan, V., Preston, A., Maskell, D.J., Field, R.A., Brisson, J.-R. and Lam, J.S.
    Erratum report. Identification and biochemical characterization of two novel UDP-2,3-diacetamido-2,3-dideoxy-α-D-glucuronic acid 2-epimerases from respiratory pathogens.
    Biochem. J. 405: 625 (2007).
  3. Sri Kannathasan, V., Staines, A.G., Dong, C.J., Field, R.A., Preston, A.G., Maskell, D.J. and Naismith, J. H.
    Overexpression, purification, crystallization and data collection on the Bordetella pertussis wlbD gene product, a putative UDP-GlcNAc 2'-epimerase.
    Acta Crystallogr. D Biol. Crystallogr. 57: 1310-1312 (2001). [PMID: 11526328]

[EC 5.1.3.23 created 2007]