EC 18.104.22.168 - Nocardicin-A epimerase
IntEnz Enzyme Nomenclature
nocJ (gene name)
Requires pyridoxal 5'-phosphate. The enzyme, characterized from the bacterium Nocardia uniformis, is involved in the biosynthesis of the monolactam antibiotic nocardicin A. It catalyses the epimerization of the amino group at position 9' from (S) configuration to (R). The enzyme can act on both isonocardicin A and isonocardicin C, but the in vivo substrate appears to be the latter .
Links to other databases
Cell-free biosynthesis of nocardicin-A from nocardicin-E and S-adenosylmethionine.J. Am. Chem. Soc. 110 : 8238-8239 (1988).
Mutational analysis and characterization of nocardicin C-9' epimerase.J. Biol. Chem. 279 : 38220-38227 (2004). [PMID: 15252031]
Mutational analysis of nocK and nocL in the nocardicin a producer Nocardia uniformis.J. Bacteriol. 187 : 739-746 (2005). [PMID: 15629944]
[EC 22.214.171.124 created 1992, modified 2016]