EC 5.1.1.14 - Nocardicin-A epimerase

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IntEnz Enzyme Nomenclature
EC 5.1.1.14

Names

Accepted name:
nocardicin-A epimerase
Other names:
isonocardicin A epimerase
nocJ (gene name)
Systematic name:
nocardicin-A epimerase

Reactions

Cofactor

Comments:

Requires pyridoxal 5'-phosphate. The enzyme, characterized from the bacterium Nocardia uniformis, is involved in the biosynthesis of the monolactam antibiotic nocardicin A. It catalyses the epimerization of the amino group at position 9' from (S) configuration to (R). The enzyme can act on both isonocardicin A and isonocardicin C, but the in vivo substrate appears to be the latter [3].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0050143

References

  1. Wilson, B.A., Bantia, S., Salituro, G.M., Reeve, A.M. and Townsend, C.A.
    Cell-free biosynthesis of nocardicin-A from nocardicin-E and S-adenosylmethionine.
    J. Am. Chem. Soc. 110: 8238-8239 (1988).
  2. Kelly, W. L., Townsend, C. A.
    Mutational analysis and characterization of nocardicin C-9' epimerase.
    J. Biol. Chem. 279: 38220-38227 (2004). [PMID: 15252031]
  3. Kelly, W. L., Townsend, C. A.
    Mutational analysis of nocK and nocL in the nocardicin a producer Nocardia uniformis.
    J. Bacteriol. 187: 739-746 (2005). [PMID: 15629944]

[EC 5.1.1.14 created 1992, modified 2016]