EC - TRNA-intron lyase

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IntEnz Enzyme Nomenclature


Accepted name:
tRNA-intron lyase
Other names:
splicing endonuclease
tRNA splicing endonuclease
tRNATRPintron endonuclease
transfer ribonucleate intron endoribonuclease
transfer splicing endonuclease
Systematic name:
pretRNA lyase (intron-removing; cyclic-2',3'-phosphate-forming)



The enzyme catalyses the final stage in the maturation of tRNA molecules. Formerly EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
CAS Registry Number: 117444-13-0
UniProtKB/Swiss-Prot: (57) [show] [UniProt]


  1. Attardi, D.G., Margarit, I. and Tocchini-Valentini, G.P.
    Structural alterations in mutant precursors of the yeast tRNALeu3 gene which behave as defective substrates for a highly purified splicing endoribonuclease.
    EMBO J. 4: 3289-3297 (1985). [PMID: 3937725]
  2. Peebles, C.L., Gegenheimer, P. and Abelson, J.
    Precise excision of intervening sequences from precursor tRNAs by a membrane-associated yeast endonuclease.
    Cell 32: 525-536 (1983). [PMID: 6186398]
  3. Thompson, L.D., Brandon, L.D., Nieuwlandt, D.T. and Daniels, C.J.
    Transfer RNA intron processing in the halophilic archaebacteria.
    Can. J. Microbiol. 35: 36-42 (1989). [PMID: 2470486]
  4. Thompson, L.D. and Daniels, C.J.
    A tRNA(Trp) intron endonuclease from Halobacterium volcanii. Unique substrate recognition properties.
    J. Biol. Chem. 263: 17951-17959 (1988). [PMID: 3192521]

[EC created 1992 as EC, transferred 2014 to EC]