EC - Glycosylphosphatidylinositol diacylglycerol-lyase

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IntEnz Enzyme Nomenclature


Accepted name:
glycosylphosphatidylinositol diacylglycerol-lyase
Other names:
(glycosyl)phosphatidylinositol-specific phospholipase C
GPI-specific phospholipase C
glycosyl inositol phospholipid anchor-hydrolyzing enzyme
glycosylphosphatidylinositol-phospholipase C
glycosylphosphatidylinositol-specific phospholipase C
variant-surface-glycoprotein phospholipase C
6-(α-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol diacylglycerol-lyase (1,2-cyclic-phosphate-forming)
Systematic name:
6-(α-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol 1,2-diacyl-sn-glycerol-lyase [6-(α-D-glucosaminyl)-1D-myo-inositol 1,2-cyclic phosphate-forming]



This enzyme is also active when O-4 of the glucosamine is substituted by carrying the oligosaccharide that can link a protein to the structure. It therefore cleaves proteins from the lipid part of the glycosylphostphatidylinositol (GPI) anchors. In some cases, the long-chain acyl group at the sn-1 position of glycerol is replaced by an alkyl or alk-1-enyl group. In other cases, the diacylglycerol is replaced by ceramide. The only characterized enzyme with this specificity is from Trypanosoma brucei, where the acyl groups are myristoyl, but the function of the trypanosome enzyme is unknown. Substitution on O-2 of the inositol blocks action of this enzyme. It is not identical with EC, glycosylphosphatidylinositol phospholipase D.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0047396
CAS Registry Number: 129070-68-4


  1. Hereld, D., Krakow, J.L., Bangs, J.D., Hart, G.W. and Englund, P.T.
    A phospholipase C from Trypanosoma brucei which selectively cleaves the glycolipid on the variant surface glycoprotein.
    J. Biol. Chem. 261: 13813-13819 (1986). [PMID: 3759991]
  2. Carnall, N., Webb, H. and Carrington, M.
    Mutagenesis study of the glycosylphosphatidylinositol phospholipase C of Trypanosoma brucei.
    Mol. Biochem. Parasitol. 90: 423-432 (1997). [PMID: 9476790]
  3. Armah, D.A. and Mensa-Wilmot, K.
    Tetramerization of glycosylphosphatidylinositol-specific phospholipase C from Trypanosoma brucei.
    J. Biol. Chem. 275: 19334-19342 (2000). [PMID: 10764777]

[EC created 1989 as EC, transferred 2002 to EC]