EC - Isoprene-epoxide—glutathione S-transferase

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IntEnz Enzyme Nomenclature


Accepted name:
isoprene-epoxide—glutathione S-transferase
Other name:
isoI (gene name)
Systematic name:
2-(glutathione-S-yl)-2-methylbut-3-en-1-ol lyase [(3R)-3,4-epoxy-3-methylbut-1-ene forming]



The enzyme, characterized from the bacterium Rhodococcus sp. AD45, is involved in isoprene degradation. The enzyme can catalyse the glutathione-dependent ring opening of various epoxides, but the highest activity is with (3R)-3,4-epoxy-3-methylbut-1-ene, which is derived from isoprene by EC, alkene monooxygenase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. van Hylckama Vlieg, J. E., Kingma, J., van den Wijngaard, A. J., Janssen, D. B.
    A glutathione S-transferase with activity towards cis-1, 2-dichloroepoxyethane is involved in isoprene utilization by Rhodococcus sp. strain AD45.
    Appl. Environ. Microbiol. 64 : 2800-2805 (1998). [PMID: 9687433]
  2. van Hylckama Vlieg, J. E., Kingma, J., Kruizinga, W., Janssen, D. B.
    Purification of a glutathione S-transferase and a glutathione conjugate-specific dehydrogenase involved in isoprene metabolism in Rhodococcus sp. strain AD45.
    J. Bacteriol. 181 : 2094-2101 (1999). [PMID: 10094686]

[EC created 2016]