EC 4.3.3.6 - Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)

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IntEnz Enzyme Nomenclature
EC 4.3.3.6

Names

Accepted name:
pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)
Other name:
PdxST
Systematic name:
D-ribose 5-phosphate,D-glyceraldehyde 3-phosphate pyridoxal 5'-phosphate-lyase

Reactions

Comments:

The ammonia is provided by the glutaminase subunit and channeled to the active site of the lyase subunit by a 100 Å tunnel. The enzyme can also use ribulose 5-phosphate and dihydroxyacetone phosphate. The enzyme complex is found in aerobic bacteria, archeae, fungi and plants.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0036381
UniProtKB/Swiss-Prot: (462) [show] [UniProt]

References

  1. Burns, K. E., Xiang, Y., Kinsland, C. L., McLafferty, F. W., Begley, T. P.
    Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway.
    J. Am. Chem. Soc. 127 : 3682-3683 (2005). [PMID: 15771487]
  2. Raschle, T., Amrhein, N., Fitzpatrick, T. B.
    On the two components of pyridoxal 5'-phosphate synthase from Bacillus subtilis.
    J. Biol. Chem. 280 : 32291-32300 (2005). [PMID: 16030023]
  3. Strohmeier, M., Raschle, T., Mazurkiewicz, J., Rippe, K., Sinning, I., Fitzpatrick, T. B., Tews, I.
    Structure of a bacterial pyridoxal 5'-phosphate synthase complex.
    Proc. Natl. Acad. Sci. USA 103 : 19284-19289 (2006). [PMID: 17159152]
  4. Raschle, T., Arigoni, D., Brunisholz, R., Rechsteiner, H., Amrhein, N., Fitzpatrick, T. B.
    Reaction mechanism of pyridoxal 5'-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate.
    J. Biol. Chem. 282 : 6098-6105 (2007). [PMID: 17189272]
  5. Hanes, J. W., Keresztes, I., Begley, T. P.
    Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5'-phosphate.
    Angew. Chem. Int. Ed. Engl. 47 : 2102-2105 (2008). [PMID: 18260082]
  6. Hanes, J. W., Burns, K. E., Hilmey, D. G., Chatterjee, A., Dorrestein, P. C., Begley, T. P.
    Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate.
    J. Am. Chem. Soc. 130 : 3043-3052 (2008). [PMID: 18271580]
  7. Hanes, J. W., Keresztes, I., Begley, T. P.
    13C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase.
    Nat. Chem. Biol. 4 : 425-430 (2008). [PMID: 18516049]
  8. Wallner, S., Neuwirth, M., Flicker, K., Tews, I., Macheroux, P.
    Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from Bacillus subtilis.
    Biochemistry 48 : 1928-1935 (2009). [PMID: 19152323]

[EC 4.3.3.6 created 2011]