EC 4.3.3.6 - Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)
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ENZYME view
IntEnz Enzyme Nomenclature
EC 4.3.3.6
Names
Accepted name:
pyridoxal 5'-phosphate synthase (glutamine hydrolyzing)
Other
name:
PdxST
Systematic name:
D-ribose 5-phosphate,D-glyceraldehyde 3-phosphate pyridoxal 5'-phosphate-lyase
Reactions
- (1) D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
- (1a) L-glutamine + H2O = L-glutamate + NH3
- (1b) D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + NH3 = pyridoxal 5'-phosphate + 4 H2O + phosphate
Comments:
The ammonia is provided by the glutaminase subunit and channeled to the active site of the lyase subunit by a 100 Å tunnel. The enzyme can also use ribulose 5-phosphate and dihydroxyacetone phosphate. The enzyme complex is found in aerobic bacteria, archeae, fungi and plants.
Links to other databases
Gene Ontology:
GO:0036381
References
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Reconstitution and biochemical characterization of a new pyridoxal-5'-phosphate biosynthetic pathway.J. Am. Chem. Soc. 127 : 3682-3683 (2005). [PMID: 15771487]
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On the two components of pyridoxal 5'-phosphate synthase from Bacillus subtilis.J. Biol. Chem. 280 : 32291-32300 (2005). [PMID: 16030023]
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Structure of a bacterial pyridoxal 5'-phosphate synthase complex.Proc. Natl. Acad. Sci. USA 103 : 19284-19289 (2006). [PMID: 17159152]
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Reaction mechanism of pyridoxal 5'-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate.J. Biol. Chem. 282 : 6098-6105 (2007). [PMID: 17189272]
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Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5'-phosphate.Angew. Chem. Int. Ed. Engl. 47 : 2102-2105 (2008). [PMID: 18260082]
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Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate.J. Am. Chem. Soc. 130 : 3043-3052 (2008). [PMID: 18271580]
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13C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase.Nat. Chem. Biol. 4 : 425-430 (2008). [PMID: 18516049]
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Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from Bacillus subtilis.Biochemistry 48 : 1928-1935 (2009). [PMID: 19152323]
[EC 4.3.3.6 created 2011]