EC - Isoprene synthase

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IntEnz Enzyme Nomenclature


Accepted name:
isoprene synthase
Other names:
Systematic name:
dimethylallyl-diphosphate diphosphate-lyase (isoprene-forming)




Requires Mg2+ or Mn2+ for activity. This enzyme is located in the chloroplast of isoprene-emitting plants, such as poplar and aspen, and may be activitated by light-dependent changes in chloroplast pH and Mg2+ concentration [2,8].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0034009


  1. Silver, G.M. and Fall, R.
    Enzymatic synthesis of isoprene from dimethylallyl diphosphate in aspen leaf extracts.
    Plant Physiol. 97: 1588-1591 (1991). [PMID: 16668590]
  2. Silver, G.M. and Fall, R.
    Characterization of aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere.
    J. Biol. Chem. 270: 13010-13016 (1995). [PMID: 7768893]
  3. Wildermuth, M.C. and Fall, R.
    Light-dependent isoprene emission (characterization of a thylakoid-bound isoprene synthase in Salix discolor chloroplasts).
    Plant Physiol. 112: 171-182 (1996). [PMID: 12226383]
  4. Schnitzler, J.P., Arenz, R., Steinbrecher, R. and Lehming, A.
    Characterization of an isoprene synthase from leaves of Quercus petraea.
    Bot. Acta 109: 216-221 (1996).
  5. Miller, B., Oschinski, C. and Zimmer, W.
    First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli.
    Planta 213: 483-487 (2001). [PMID: 11506373]
  6. Sivy, T.L., Shirk, M.C. and Fall, R.
    Isoprene synthase activity parallels fluctuations of isoprene release during growth of Bacillus subtilis.
    Biochem. Biophys. Res. Commun. 294: 71-75 (2002). [PMID: 12054742]
  7. Sasaki, K., Ohara, K. and Yazaki, K.
    Gene expression and characterization of isoprene synthase from Populus alba.
    FEBS Lett. 579: 2514-2518 (2005). [PMID: 15848197]
  8. Schnitzler, J.P., Zimmer, I., Bachl, A., Arend, M., Fromm, J. and Fischbach, R.J.
    Biochemical properties of isoprene synthase in poplar (Populus x canescens).
    Planta 222: 777-786 (2005). [PMID: 16052321]

[EC created 2007]