EC 4.2.3.18 - Abieta-7,13-diene synthase

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IntEnz Enzyme Nomenclature
EC 4.2.3.18

Names

Accepted name:
abieta-7,13-diene synthase
Other names:
copalyl-diphosphate diphosphate-lyase (cyclizing) [ambiguous]
abietadiene cyclase [ambiguous]
abietadiene synthase [ambiguous]
Systematic name:
(+)-copalyl-diphosphate diphosphate-lyase [cyclizing, abieta-7,13-diene-forming]

Reaction

Cofactor

Comments:

Part of a bifunctional enzyme involved in the biosynthesis of abietadiene. See also EC 5.5.1.12, copalyl diphosphate synthase. Requires Mg+.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0050554
CAS Registry Number: 157972-08-2
UniProtKB/Swiss-Prot:

References

  1. Peters, R.J., Flory, J.E., Jetter, R., Ravn, M.M., Lee, H.J., Coates, R.M. and Croteau, R.B.
    Abietadiene synthase from grand fir (Abies grandis): characterization and mechanism of action of the "pseudomature" recombinant enzyme.
    Biochemistry 39: 15592-15602 (2000). [PMID: 11112547]
  2. Peters, R.J., Ravn, M.M., Coates, R.M. and Croteau, R.B.
    Bifunctional abietadiene synthase: free diffusive transfer of the (+)-copalyl diphosphate intermediate between two distinct active sites.
    J. Am. Chem. Soc. 123: 8974-8978 (2001). [PMID: 11552804]
  3. Peters, R.J. and Croteau, R.B.
    Abietadiene synthase catalysis: mutational analysis of a prenyl diphosphate ionization-initiated cyclization and rearrangement.
    Proc. Natl. Acad. Sci. USA 99: 580-584 (2002). [PMID: 11805316]
  4. Peters, R.J. and Croteau, R.B.
    Abietadiene synthase catalysis: conserved residues involved in protonation-initiated cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate.
    Biochemistry 41: 1836-1842 (2002). [PMID: 11827528]
  5. Ravn, M.M., Peters, R.J., Coates, R.M. and Croteau, R.
    Mechanism of abietadiene synthase catalysis: stereochemistry and stabilization of the cryptic pimarenyl carbocation intermediates.
    J. Am. Chem. Soc. 124: 6998-7006 (2002). [PMID: 12059223]

[EC 4.2.3.18 created 2002, modified 2012]