EC - (5-formylfuran-3-yl)methyl phosphate synthase

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IntEnz Enzyme Nomenclature


Accepted name:
(5-formylfuran-3-yl)methyl phosphate synthase
Other names:
mfnB (gene name)
4-HFC-P synthase
2-furaldehyde phosphate synthase
Systematic name:
D-glyceraldehyde-3-phosphate phosphate-lyase [D-glyceraldehyde-3-phosphate-adding (5-formylfuran-3-yl)methyl-phosphate-forming]



The enzyme catalyses the reaction in the direction of producing (5-formylfuran-3-yl)methyl phosphate, an intermediate in the biosynthesis of methanofuran. The sequence of events starts with the removal of a phosphate group, followed by aldol condensation and cyclization. Methanofuran is a carbon-carrier cofactor involved in the first step of the methanogenic reduction of carbon dioxide by methanogenic archaea. Formerly EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot: (18) [show] [UniProt]


  1. Miller, D., Wang, Y., Xu, H., Harich, K., White, R. H.
    Biosynthesis of the 5-(Aminomethyl)-3-furanmethanol moiety of methanofuran.
    Biochemistry 53 : 4635-4647 (2014). [PMID: 24977328]
  2. Bobik, T. A., Morales, E. J., Shin, A., Cascio, D., Sawaya, M. R., Arbing, M., Yeates, T. O., Rasche, M. E.
    Structure of the methanofuran/methanopterin-biosynthetic enzyme MJ1099 from Methanocaldococcus jannaschii.
    Acta Crystallogr F Struct Biol Commun 70 : 1472-1479 (2014). [PMID: 25372812]
  3. Wang, Y., Jones, M. K., Xu, H., Ray, W. K., White, R. H.
    Mechanism of the Enzymatic Synthesis of 4-(Hydroxymethyl)-2- furancarboxaldehyde-phosphate (4-HFC-P) from Glyceraldehyde-3-phosphate Catalyzed by 4-HFC-P Synthase.
    Biochemistry 54 : 2997-3008 (2015). [PMID: 25905665]

[EC created 2015 as EC, transferred 2015 to EC]