EC 4.2.2.26 - Oligo-alginate lyase

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IntEnz Enzyme Nomenclature
EC 4.2.2.26

Names

Accepted name:
oligo-alginate lyase
Other names:
aly (gene name) [ambiguous]
oalS17 (gene name)
oligoalginate lyase
exo-oligoalginate lyase
Systematic name:
alginate oligosaccharide 4-deoxy-α-L-erythro-hex-4-enopyranuronate-(1→4)-hexananopyranuronate lyase

Reaction

Comments:

The enzyme degrades unsaturated oligosaccharides produced by the action of alginate lyases (EC 4.2.2.3 and EC 4.2.2.11) on alginate, by repeatedly removing the unsaturated residue from the non-reducing end until only unsaturated monosaccharides are left. The enzyme catalyses a β-elimination reaction, generating a new unsaturated non-reducing end after removal of the pre-existing one.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0052764
UniProtKB/Swiss-Prot:

References

  1. Hashimoto, W., Miyake, O., Momma, K., Kawai, S., Murata, K.
    Molecular identification of oligoalginate lyase of Sphingomonas sp. strain A1 as one of the enzymes required for complete depolymerization of alginate.
    J. Bacteriol. 182 : 4572-4577 (2000). [PMID: 10913091]
  2. Kim, H. T., Chung, J. H., Wang, D., Lee, J., Woo, H. C., Choi, I. G., Kim, K. H.
    Depolymerization of alginate into a monomeric sugar acid using Alg17C, an exo-oligoalginate lyase cloned from Saccharophagus degradans 2-40.
    Appl. Microbiol. Biotechnol. 93 : 2233-2239 (2012). [PMID: 22281843]
  3. Jagtap, S. S., Hehemann, J. H., Polz, M. F., Lee, J. K., Zhao, H.
    Comparative biochemical characterization of three exolytic oligoalginate lyases from Vibrio splendidus reveals complementary substrate scope, temperature, and pH adaptations.
    Appl. Environ. Microbiol. 80 : 4207-4214 (2014). [PMID: 24795372]
  4. Wang, L., Li, S., Yu, W., Gong, Q.
    Cloning, overexpression and characterization of a new oligoalginate lyase from a marine bacterium, Shewanella sp.
    Biotechnol. Lett. 37 : 665-671 (2015). [PMID: 25335746]

[EC 4.2.2.26 created 2015]