EC 4.2.2.23 - Rhamnogalacturonan endolyase

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IntEnz Enzyme Nomenclature
EC 4.2.2.23

Names

Accepted name:
rhamnogalacturonan endolyase
Other names:
rhamnogalacturonase B
α-L-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronide lyase
Rgase B
rhamnogalacturonan α-L-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronide lyase
RG-lyase
YesW
RGL4
Rgl11A
Rgl11Y
RhiE
Systematic name:
α-L-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronate endolyase

Reaction

Comments:

The enzyme is part of the degradation system for rhamnogalacturonan I in Bacillus subtilis strain 168 and Aspergillus aculeatus.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0102210
UniProtKB/Swiss-Prot: (24) [show] [UniProt]

References

  1. Mutter, M., Colquhoun, I. J., Schols, H. A., Beldman, G., Voragen, A. G.
    Rhamnogalacturonase B from Aspergillus aculeatus is a rhamnogalacturonan α-L-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronide lyase.
    Plant Physiol. 110 : 73-77 (1996). [PMID: 8587995]
  2. Azadi, P., O'Neill, M. A., Bergmann, C., Darvill, A. G., Albersheim, P.
    The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase.
    Glycobiology 5 : 783-789 (1995). [PMID: 8720076]
  3. Mutter, M., Colquhoun, I. J., Beldman, G., Schols, H. A., Bakx, E. J., Voragen, A. G.
    Characterization of recombinant rhamnogalacturonan α-L-rhamnopyranosyl-(1→4)-α-D-galactopyranosyluronide lyase from Aspergillus aculeatus. An enzyme that fragments rhamnogalacturonan I regions of pectin.
    Plant Physiol. 117 : 141-152 (1998). [PMID: 9576783]
  4. Kadirvelraj, R., Harris, P., Poulsen, J. C., Kauppinen, S., Larsen, S.
    A stepwise optimization of crystals of rhamnogalacturonan lyase from Aspergillus aculeatus.
    Acta Crystallogr. D Biol. Crystallogr. 58 : 1346-1349 (2002). [PMID: 12136151]
  5. Laatu, M., Condemine, G.
    Rhamnogalacturonate lyase RhiE is secreted by the out system in Erwinia chrysanthemi.
    J. Bacteriol. 185 : 1642-1649 (2003). [PMID: 12591882]
  6. Pages, S., Valette, O., Abdou, L., Belaich, A., Belaich, J. P.
    A rhamnogalacturonan lyase in the Clostridium cellulolyticum cellulosome.
    J. Bacteriol. 185 : 4727-4733 (2003). [PMID: 12896991]
  7. Ochiai, A., Yamasaki, M., Itoh, T., Mikami, B., Hashimoto, W., Murata, K.
    Crystallization and preliminary X-ray analysis of the rhamnogalacturonan lyase YesW from Bacillus subtilis strain 168, a member of polysaccharide lyase family 11.
    Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62 : 438-440 (2006). [PMID: 16682770]
  8. Jensen, M. H., Otten, H., Christensen, U., Borchert, T. V., Christensen, L. L., Larsen, S., Leggio, L. L.
    Structural and biochemical studies elucidate the mechanism of rhamnogalacturonan lyase from Aspergillus aculeatus.
    J. Mol. Biol. 404 : 100-111 (2010). [PMID: 20851126]

[EC 4.2.2.23 created 2011]