EC 4.2.1.80 - 2-oxopent-4-enoate hydratase
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 4.2.1.80
Names
Accepted name:
2-oxopent-4-enoate hydratase
Other
names:
2-keto-4-pentenoate hydratase
2-keto-4-pentenoate (vinylpyruvate)hydratase
OEH
4-hydroxy-2-oxopentanoate hydro-lyase
mhpD (gene name)
4-hydroxy-2-oxopentanoate hydro-lyase (2-oxopent-4-enoate-forming)
ahdF (gene name)
todG (gene name)
cmtF (gene name)
xylJ (gene name)
cnbE (gene name)
2-keto-4-pentenoate (vinylpyruvate)hydratase
OEH
4-hydroxy-2-oxopentanoate hydro-lyase
mhpD (gene name)
4-hydroxy-2-oxopentanoate hydro-lyase (2-oxopent-4-enoate-forming)
ahdF (gene name)
todG (gene name)
cmtF (gene name)
xylJ (gene name)
cnbE (gene name)
Systematic name:
(S)-4-hydroxy-2-oxopentanoate hydro-lyase ((2Z)-2-hydroxypenta-2,4-dienoate-forming)
Reaction
- (S)-4-hydroxy-2-oxopentanoate = (2Z)-2-hydroxypenta-2,4-dienoate + H2O
Comments:
The enzyme is involved in the catechol meta-cleavage pathway, a major mechanism for degradation of aromatic compounds. Also acts, more slowly, on cis-2-oxohex-4-enoate, but not on the trans-isomer. The enzyme was named when it was thought that the substrate is 2-oxopent-4-enoate. However, it was later found that the actual substrate is its tautomer (2Z)-2-hydroxypenta-2,4-dienoate. In some organisms the enzyme forms a complex with EC 4.1.1.77, 2-oxo-3-hexenedioate decarboxylase (previously named 4-oxalocrotonate decarboxylase).
Links to other databases
Enzymes and pathways:
NC-IUBMB
,
BRENDA
,
DIAGRAM
,
DIAGRAM
,
DIAGRAM
,
ExplorEnz
,
ENZYME@ExPASy
,
KEGG
,
MetaCyc
,
UM-BBD
,
UniPathway
Gene Ontology:
GO:0008684
CAS Registry Numbers:
227181-25-1
229017-66-7
64427-80-1
References
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Metabolism of allylglycine and cis-crotylglycine by Pseudomonas putida (arvilla) mt-2 harboring a TOL plasmid.J. Bacteriol. 148 : 72-82 (1981). [PMID: 7287632]
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Physically associated enzymes produce and metabolize 2-hydroxy-2,4-dienoate, a chemically unstable intermediate formed in catechol metabolism via meta cleavage in Pseudomonas putida.J. Bacteriol. 171 : 6251-6258 (1989). [PMID: 2681159]
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Purification, characterisation and reaction mechanism of monofunctional 2-hydroxypentadienoic acid hydratase from Escherichia coli.Eur. J. Biochem. 251 : 98-106 (1998). [PMID: 9492273]
[EC 4.2.1.80 created 1984]