EC - 2-methylcitrate dehydratase

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IntEnz Enzyme Nomenclature


Accepted name:
2-methylcitrate dehydratase
Other names:
2-methylcitrate hydro-lyase
2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase
Systematic name:
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase [(Z)-but-2-ene-1,2,3-tricarboxylate-forming]



The enzyme is specific for (2S,3S)-methylcitrate, showing no activity with (2R,3S)-methylcitrate [2]. The enzyme can also use cis-aconitate as a substrate but more slowly [2]. Both this enzyme and EC, aconitate hydratase, are required to complete the isomerization of (2S,3S)-methylcitrate to (2R,3S)-2-methylisocitrate [2].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0047547
CAS Registry Number: 80891-26-5
UniProtKB/Swiss-Prot: (12) [show] [UniProt]


  1. Aoki, H. and Tabuchi, T.
    Purification and properties of 2-methylcitrate dehydratase from Yarrowia lipolytica.
    Agric. Biol. Chem. 45 : 2831-2837 (1981).
  2. Brock, M., Maerker, C., Schütz, A., Völker, U., Buckel, W.
    Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase.
    Eur. J. Biochem. 269 : 6184-6194 (2002). [PMID: 12473114]

[EC created 1984, modified 2007]