EC 22.214.171.124 - 2-methylcitrate dehydratase
IntEnz view ENZYME view
IntEnz Enzyme Nomenclature
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase [(Z)-but-2-ene-1,2,3-tricarboxylate-forming]
- (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O
The enzyme is specific for (2S,3S)-methylcitrate, showing no activity with (2R,3S)-methylcitrate . The enzyme can also use cis-aconitate as a substrate but more slowly . Both this enzyme and EC 126.96.36.199, aconitate hydratase, are required to complete the isomerization of (2S,3S)-methylcitrate to (2R,3S)-2-methylisocitrate .
Links to other databases
Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Gene Ontology: GO:0047547
CAS Registry Number: 80891-26-5
Purification and properties of 2-methylcitrate dehydratase from Yarrowia lipolytica.Agric. Biol. Chem. 45 : 2831-2837 (1981).
Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase.Eur. J. Biochem. 269 : 6184-6194 (2002). [PMID: 12473114]
[EC 188.8.131.52 created 1984, modified 2007]