EC - Short-chain-enoyl-CoA hydratase

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IntEnz Enzyme Nomenclature


Accepted name:
short-chain-enoyl-CoA hydratase
Other names:
3-hydroxybutyryl-CoA dehydratase
crt (gene name)
Systematic name:
short-chain-(3S)-3-hydroxyacyl-CoA hydro-lyase



The enzyme from the bacterium Clostridium acetobutylicum is part of the central fermentation pathway and plays a key role in the production of both acids and solvents. It is specific for short, C4-C6, chain length substrates and exhibits an extremely high turnover number for crotonyl-CoA. cf. EC, enoyl-CoA hydratase and EC, long-chain-enoyl-CoA hydratase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. Waterson, R. M., Castellino, F. J., Hass, G. M., Hill, R. L.
    Purification and characterization of crotonase from Clostridium acetobutylicum.
    J. Biol. Chem. 247 : 5266-5271 (1972). [PMID: 5057466]
  2. Waterson, R. M., Conway, R. S.
    Enoyl-CoA hydratases from Clostridium acetobutylicum and Escherichia coli.
    Meth. Enzymol. 71 Pt C : 421-430 (1981). [PMID: 7024731]
  3. Boynton, Z. L., Bennet, G. N., Rudolph, F. B.
    Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824.
    J. Bacteriol. 178 : 3015-3024 (1996). [PMID: 8655474]

[EC created 2014]