EC 4.2.1.121 - Colneleate synthase

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IntEnz Enzyme Nomenclature
EC 4.2.1.121

Names

Accepted name:
colneleate synthase
Other names:
9-divinyl ether synthase
9-DES
CYP74D
CYP74D1
CYP74 cytochrome P-450
DES1
(8E)-9-[(1E,3E)-nona-1,3-dien-1-yloxy]non-8-enoate synthase
Systematic name:
(9S,10E,12Z)-9-hydroperoxyoctadeca-10,12-dienoate hydro-lyase

Reactions

Cofactor

Comments:

A heme-thiolate protein (P450) [2]. It catalyses the selective removal of pro-R hydrogen at C-8 in the biosynthesis of colneleic acid [4]. It forms also (8E)-9-[(1E,3Z,6Z)-nona-1,3,6-trien-1-yloxy]non-8-enoic acid (i.e. colnelenate) from (9Z,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate. The corresponding 13-hydroperoxides are poor substrates [1,3]. The divinyl ethers colneleate and colnelenate have antimicrobial activity.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0102895
UniProtKB/Swiss-Prot:

References

  1. Stumpe, M., Kandzia, R., Göbel, C., Rosahl, S., Feussner, I.
    A pathogen-inducible divinyl ether synthase (CYP74D) from elicitor-treated potato suspension cells.
    FEBS Lett. 507: 371-376 (2001). [PMID: 11696374]
  2. Itoh, A., Howe, G. A.
    Molecular cloning of a divinyl ether synthase. Identification as a CYP74 cytochrome P-450.
    J. Biol. Chem. 276: 3620-3627 (2001). [PMID: 11060314]
  3. Fammartino, A., Cardinale, F., Gobel, C., Mene-Saffrane, L., Fournier, J., Feussner, I., Esquerre-Tugaye, M. T.
    Characterization of a divinyl ether biosynthetic pathway specifically associated with pathogenesis in tobacco.
    Plant Physiol. 143: 378-388 (2007). [PMID: 17085514]
  4. Hamberg, M.
    Hidden stereospecificity in the biosynthesis of divinyl ether fatty acids.
    FEBS J. 272: 736-743 (2005). [PMID: 15670154]

[EC 4.2.1.121 created 2010]