EC - Enoyl-CoA hydratase 2

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IntEnz Enzyme Nomenclature


Accepted name:
enoyl-CoA hydratase 2
Other names:
2-enoyl-CoA hydratase 2
D-3-hydroxyacyl-CoA hydro-lyase
D-specific 2-trans-enoyl-CoA hydratase
Systematic name:
(3R)-3-hydroxyacyl-CoA hydro-lyase



This enzyme catalyses a hydration step in peroxisomal β-oxidation. The human multifunctional enzyme type 2 (MFE-2) is a 79000 Da enzyme composed of three functional units: (3R)-hydroxyacyl-CoA dehydrogenase, 2-enoyl-CoA hydratase 2 and sterol carrier protein 2-like units [1]. The enzymes from Aeromonas caviae [4] and Arabidopsis thaliana [5] are monofunctional enzymes. 2-Enoyl-CoA hydratase 3 from Candida tropicalis is a part from multifunctional enzyme type 2 [3].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot: (11) [show] [UniProt]


  1. Koski, K. M., Haapalainen, A. M., Hiltunen, J. K., Glumoff, T.
    Crystal structure of 2-enoyl-CoA hydratase 2 from human peroxisomal multifunctional enzyme type 2.
    J. Mol. Biol. 345: 1157-1169 (2005). [PMID: 15644212]
  2. Fukui, T., Shiomi, N., Doi, Y.
    Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae.
    J. Bacteriol. 180: 667-673 (1998). [PMID: 9457873]
  3. Koski, M. K., Haapalainen, A. M., Hiltunen, J. K., Glumoff, T.
    Crystallization and preliminary crystallographic data of 2-enoyl-CoA hydratase 2 domain of Candida tropicalis peroxisomal multifunctional enzyme type 2.
    Acta Crystallogr. D Biol. Crystallogr. 59: 1302-1305 (2003). [PMID: 12832794]
  4. Hisano, T., Fukui, T., Iwata, T., Doi, Y.
    Crystallization and preliminary X-ray analysis of (R)-specific enoyl-CoA hydratase from Aeromonas caviae involved in polyhydroxyalkanoate biosynthesis.
    Acta Crystallogr. D Biol. Crystallogr. 57: 145-147 (2001). [PMID: 11134939]
  5. Goepfert, S., Hiltunen, J. K., Poirier, Y.
    Identification and functional characterization of a monofunctional peroxisomal enoyl-CoA hydratase 2 that participates in the degradation of even cis-unsaturated fatty acids in Arabidopsis thaliana.
    J. Biol. Chem. 281: 35894-35903 (2006). [PMID: 16982622]
  6. Engeland, K., Kindl, H.
    Evidence for a peroxisomal fatty acid β-oxidation involving D-3-hydroxyacyl-CoAs. Characterization of two forms of hydro-lyase that convert D-(−)-3-hydroxyacyl-CoA into 2-trans-enoyl-CoA.
    Eur. J. Biochem. 200: 171-178 (1991). [PMID: 1879422]

[EC created 2009]