EC 4.2.1.117 - 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming)

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IntEnz Enzyme Nomenclature
EC 4.2.1.117

Names

Accepted name:
2-methylcitrate dehydratase (2-methyl-trans-aconitate forming)
Systematic name:
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase (2-methyl-trans-aconitate forming)

Reaction

Cofactor

Comments:

Catalyses the dehydration of (2S,3S)-2-methylcitrate, forming the trans isomer of 2-methyl-aconitate (unlike EC 4.2.1.79, which forms only the cis isomer). Part of a propionate degradation pathway. The enzyme from Shewanella oneidensis can also accept citrate and cis-aconitate, but activity with (2S,3S)-2-methylcitrate was approximately 2.5-fold higher. 2-methylisocitrate and isocitrate were not substrates [1]. An iron-sulfur protein.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot:

References

  1. Grimek, T. L., Escalante-Semerena, J. C.
    The acnD genes of Shewenella oneidensis and Vibrio cholerae encode a new Fe/S-dependent 2-methylcitrate dehydratase enzyme that requires prpF function in vivo.
    J. Bacteriol. 186 : 454-462 (2004). [PMID: 14702315]

[EC 4.2.1.117 created 2009]