EC 22.214.171.124 - Methanogen homoaconitase
IntEnz Enzyme Nomenclature
This enzyme catalyses several reactions in the pathway of coenzyme-B biosynthesis in methanogenic Archaea. Requires a [4Fe-4S] cluster for activity. In contrast to EC 126.96.36.199, homoaconitate hydratase, this enzyme can catalyse both the dehydration of (R)-homocitrate to form cis-homoaconitate and the subsequent hydration reaction that forms homoisocitrate. In addition to cis-homoaconitate, the enzyme can also catalyse the hydration of the physiological substrates dihomocitrate and trihomocitrate as well as the non-physiological substrate tetrahomocitrate. cis-Aconitate and threo-DL-isocitrate cannot act as substrates, and (S)-homocitrate and trans-homoaconitate act as inhibitors of the enzyme.
Links to other databases
Methanogen homoaconitase catalyzes both hydrolyase reactions in coenzyme B biosynthesis.J. Biol. Chem. 283 : 28888-28896 (2008). [PMID: 18765671]
[EC 188.8.131.52 created 2009]