EC 4.2.1.111 - 1,5-anhydro-D-fructose dehydratase

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IntEnz Enzyme Nomenclature
EC 4.2.1.111

Names

Accepted name:
1,5-anhydro-D-fructose dehydratase
Other names:
1,5-anhydro-D-fructose 4-dehydratase
1,5-anhydro-D-fructose hydrolyase
1,5-anhydro-D-arabino-hex-2-ulose dehydratase
AFDH
AF dehydratase
1,5-anhydro-D-fructose hydro-lyase
Systematic name:
1,5-anhydro-D-fructose hydro-lyase (ascopyrone-M-forming)

Reaction

Cofactor

Comments:

This enzyme catalyses one of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose [1,2]. The other enzymes involved in this pathway are EC 4.2.1.110 (aldos-2-ulose dehydratase), EC 4.2.2.13 [exo-(1→4)-α-D-glucan lyase] and EC 5.3.2.7 (ascopyrone tautomerase). Requires divalent (Ca2+ or Mg2+) or monovalent cations (Na+) for optimal activity. Unlike EC 4.2.1.110, aldos-2-ulose dehydratase, the enzyme is specific for 1,5-anhydro-D-fructose as substrate and shows no activity towards aldose-2-uloses such as 2-dehydroglucose [1,2,3]. In addition, it is inhibited by its end-product ascopyrone M [2] and it cannot convert the end-product ascopyrone M into microthecin, as can EC 4.2.1.110.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0033992

References

  1. Yu, S., Refdahl, C. and Lundt, I.
    Enzymatic description of the anhydrofructose pathway of glycogen degradation. I. Identification and purification of anhydrofructose dehydratase, ascopyrone tautomerase and α-1,4-glucan lyase in the fungus Anthracobia melaloma.
    Biochim. Biophys. Acta 1672 : 120-129 (2004). [PMID: 15110094]
  2. Yu, S. and Fiskesund, R.
    The anhydrofructose pathway and its possible role in stress response and signaling.
    Biochim. Biophys. Acta 1760 : 1314-1322 (2006). [PMID: 16822618]
  3. Yu, S.
    Enzymatic description of the anhydrofructose pathway of glycogen degradation II. Gene identification and characterization of the reactions catalyzed by aldos-2-ulose dehydratase that converts 1,5-anhydro-D-fructose to microthecin with ascopyrone M as the intermediate.
    Biochim. Biophys. Acta 1723 : 63-73 (2005). [PMID: 15716041]

[EC 4.2.1.111 created 2006]