IntEnz

EC 4.2.1.110 - Aldos-2-ulose dehydratase

IntEnz Enzyme Nomenclature
EC 4.2.1.110

Names

Reaction

• 12100 [IUBMB]
  1,5-anhydro-D-fructose

  1,5-anhydro-D-fructose

  Name origin: UniProt - CHECKED (C)

  CHEBI:16715

  Formula: C6H10O5
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  =

  H₂O

  H2O

  Name origin: UniProt - CHECKED (C)

  CHEBI:15377

  Formula: H2O
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  +

  microthecin

  microthecin

  Name origin: UniProt - CHECKED (C)

  CHEBI:51835

  Formula: C6H8O4
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

Comments:

This enzyme catalyses two of the steps in the anhydrofructose pathway, which leads to the degradation of glycogen and starch via 1,5-anhydro-D-fructose [1,2]. Aldose-2-uloses such as 2-dehydroglucose can also act as substrates, but more slowly [1,2,4]. This is a bifunctional enzyme that acts as both a lyase and as an isomerase [2]. Differs from EC 4.2.1.111, which can carry out only reaction (1a), is inhibited by its product and requires metal ions for activity [1].

Links to other databases

References

1. Yu, S. and Fiskesund, R.
   The anhydrofructose pathway and its possible role in stress response and signaling.
   Biochim. Biophys. Acta 1760 : 1314-1322 (2006). [PMID: 16822618]
2. Yu, S.
   Enzymatic description of the anhydrofructose pathway of glycogen degradation II. Gene identification and characterization of the reactions catalyzed by aldos-2-ulose dehydratase that converts 1,5-anhydro-D-fructose to microthecin with ascopyrone M as the intermediate.
   Biochim. Biophys. Acta 1723 : 63-73 (2005). [PMID: 15716041]
3. Broberg, A., Kenne, L. and Pedersen, M.
   Presence of microthecin in the red alga Gracilariopsis lemaneiformis and its formation from 1,5-anhydro-D-fructose.
   Phytochemistry 41 : 151-154 (1996).
4. Gabriel, J., Volc, J., Sedmera, P., Daniel, G. and Kubátová, E.
   Pyranosone dehydratase from the basidiomycete Phanerochaete chrysosporium: improved purification, and identification of 6-deoxy-D-glucosone and D-xylosone reaction products.
   Arch. Microbiol. 160 : 27-34 (1993). [PMID: 8352649]
5. Yu, S., Refdahl, C. and Lundt, I.
   Enzymatic description of the anhydrofructose pathway of glycogen degradation. I. Identification and purification of anhydrofructose dehydratase, ascopyrone tautomerase and α-1,4-glucan lyase in the fungus Anthracobia melaloma.
   Biochim. Biophys. Acta 1672 : 120-129 (2004). [PMID: 15110094]

[EC 4.2.1.110 created 2006]