EC 4.2.1.104 - Cyanase
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 4.2.1.104
Names
Accepted name:
cyanase
Other
names:
cyanate hydratase
cyanate aminohydrolase
cyanate hydrolase
cyanate lyase
cyanate C-N-lyase
cyanate aminohydrolase
cyanate hydrolase
cyanate lyase
cyanate C-N-lyase
Systematic name:
carbamate hydro-lyase
Reactions
- (1) cyanate + HCO3- + 2 H+ = NH3 + 2 CO2
- (1a) cyanate + HCO3 + H+ = carbamate + CO2
- (1b) carbamate + H+ = NH3 + CO2 (spontaneous)
Comments:
This enzyme, which is found in bacteria and plants, is used to decompose cyanate, which can be used as the sole source of nitrogen [6,7]. Reaction (1) can be considered as the reverse of 'carbamate = cyanate + H2O', where this is assisted by reaction with bicarbonate and carbon dioxide (see mechanism above) [2], and hence is classified in sub-subclass 4.2.1. Bicarbonate functions as a recycling substrate [2].
Links to other databases
Enzymes and pathways:
NC-IUBMB
,
BRENDA
,
DIAGRAM
,
ExplorEnz
,
ENZYME@ExPASy
,
KEGG
,
MetaCyc
,
UniPathway
Gene Ontology:
GO:0008824
CAS Registry Number:
37289-24-0
References
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Purification and properties of the inducible enzyme cyanase.Biochemistry 19 : 2882-2888 (1980). [PMID: 6994799]
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Bicarbonate is a recycling substrate for cyanase.J. Biol. Chem. 262 : 9021-9025 (1987). [PMID: 3110153]
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The synthesis of the induced enzyme, "cyanase", in E. coli.Biochim. Biophys. Acta 44 : 510-519 (1960). [PMID: 13775509]
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Some properties of the induced enzyme cyanase.Can. J. Biochem. 43 : 1063-1069 (1965). [PMID: 5322950]
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Reaction of the N-terminal methionine residues in cyanase with diethylpyrocarbonate.Biochemistry 33 : 14121-14125 (1994). [PMID: 7947823]
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Role of bicarbonate/CO2 in the inhibition of Escherichia coli growth by cyanate.J. Bacteriol. 177 : 3213-3219 (1995). [PMID: 7768821]
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Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site.Structure 8 : 505-514 (2000). [PMID: 10801492]
[EC 4.2.1.104 created 1972 as EC 3.5.5.3, transferred 1990 to EC 4.3.99.1, transferred 2001 to EC 4.2.1.104, modified 2007]