EC 184.108.40.206 - 3-hexulose-6-phosphate synthase
IntEnz Enzyme Nomenclature
3-hexulose phosphate synthase
25201 [IUBMB]D-ribulose 5-phosphateD-ribulose 5-phosphateName origin: UniProt - CHECKED (C)Formula: C5H9O8P
Charge: -2ChEBI compound status: CHECKED (C)formaldehydeformaldehydeName origin: UniProt - CHECKED (C)Formula: CH2O
Charge: 0ChEBI compound status: CHECKED (C)=
Requires Mg2+ or Mn2+ for maximal activity . The enzyme is specific for formaldehyde and D-ribulose 5-phosphate as substrates. Ribose 5-phosphate, xylulose 5-phosphate, allulose 6-phosphate and fructose 6-phosphate cannot act as substrate. This enzyme, along with EC 220.127.116.11, 6-phospho-3-hexuloisomerase, plays a key role in the ribulose-monophosphate cycle of formaldehyde fixation, which is present in many microorganisms that are capable of utilizing C1-compounds . The hyperthermophilic and anaerobic archaeon Pyrococcus horikoshii OT3 constitutively produces a bifunctional enzyme that sequentially catalyses the reactions of this enzyme and EC 18.104.22.168, 6-phospho-3-hexuloisomerase . This enzyme is a member of the orotidine 5'-monophosphate decarboxylase (OMPDC) suprafamily .
Links to other databases
Purification and properties of 3-hexulose phosphate synthase and phospho-3-hexuloisomerase from Methylococcus capsulatus.Biochem. J. 144 : 477-486 (1974). [PMID: 4219834]
3-Hexulosephosphate synthase from Methylomonas aminofaciens 77a. Purification, properties and kinetics.Biochim. Biophys. Acta 523 : 236-244 (1978). [PMID: 564713]
Cloning and sequence analysis of the gene encoding 3-hexulose-6-phosphate synthase from the methylotrophic bacterium, Methylomonas aminofaciens 77a, and its expression in Escherichia coli.FEMS Microbiol. Lett. 135 : 201-205 (1996). [PMID: 8595859]
Assimilation, dissimilation, and detoxification of formaldehyde, a central metabolic intermediate of methylotrophic metabolism.Chem. Rec. 5 : 367-375 (2005). [PMID: 16278835]
The physiological role of the ribulose monophosphate pathway in bacteria and archaea.Biosci. Biotechnol. Biochem. 70 : 10-21 (2006). [PMID: 16428816]
The archaeon Pyrococcus horikoshii possesses a bifunctional enzyme for formaldehyde fixation via the ribulose monophosphate pathway.J. Bacteriol. 187 : 3636-3642 (2005). [PMID: 15901685]
3-Hexulose phosphate pynthase from a new facultative methylotroph, Mycobacterium gastri MB19.Agric. Biol. Chem. 52 : 2659-2661 (1988).
[EC 22.214.171.124 created 2008]