EC 4.1.1.77 - 2-oxo-3-hexenedioate decarboxylase

  IntEnz view ENZYME view
XML

IntEnz Enzyme Nomenclature
EC 4.1.1.77

Names

Accepted name:
2-oxo-3-hexenedioate decarboxylase
Other names:
4-oxalocrotonate carboxy-lyase [misleading]
4-oxalocrotonate decarboxylase [misleading]
cnbF (gene name)
praD (gene name)
amnE (gene name)
nbaG (gene name)
xylI (gene name)
Systematic name:
(3E)-2-oxohex-3-enedioate carboxy-lyase (2-oxopent-4-enoate-forming)

Reaction

Comments:

Involved in the meta-cleavage pathway for the degradation of phenols, modified phenols and catechols. The enzyme has been reported to accept multiple tautomeric forms [1-4]. However, careful analysis of the stability of the different tautomers, as well as characterization of the enzyme that produces its substrate, EC 5.3.2.6, 2-hydroxymuconate tautomerase, showed that the actual substrate for the enzyme is (3E)-2-oxohex-3-enedioate [4].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0047437
CAS Registry Number: 37325-55-6
UniProtKB/Swiss-Prot:

References

  1. Shingler, V., Marklund, U., Powlowski, J.
    Nucleotide sequence and functional analysis of the complete phenol/3,4-dimethylphenol catabolic pathway of Pseudomonas sp. strain CF600.
    J. Bacteriol. 174 : 711-724 (1992). [PMID: 1732207]
  2. Takenaka, S., Murakami, S., Shinke, R., Aoki, K.
    Metabolism of 2-aminophenol by Pseudomonas sp. AP-3: modified meta-cleavage pathway.
    Arch. Microbiol. 170 : 132-137 (1998). [PMID: 9683650]
  3. Stanley, T. M., Johnson, W. H., Burks, E. A., Whitman, C. P., Hwang, C. C., Cook, P. F.
    Expression and stereochemical and isotope effect studies of active 4-oxalocrotonate decarboxylase.
    Biochemistry 39 : 718-726 (2000). [PMID: 10651637]
  4. Wang, S. C., Johnson, W. H., Czerwinski, R. M., Stamps, S. L., Whitman, C. P.
    Kinetic and stereochemical analysis of YwhB, a 4-oxalocrotonate tautomerase homologue in Bacillus subtilis: mechanistic implications for the YwhB- and 4-oxalocrotonate tautomerase-catalyzed reactions.
    Biochemistry 46 : 11919-11929 (2007). [PMID: 17902707]
  5. Kasai, D., Fujinami, T., Abe, T., Mase, K., Katayama, Y., Fukuda, M., Masai, E.
    Uncovering the protocatechuate 2,3-cleavage pathway genes.
    J. Bacteriol. 191 : 6758-6768 (2009). [PMID: 19717587]

[EC 4.1.1.77 created 1999, modified 2011, modified 2012]