EC - cis-aconitate decarboxylase

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IntEnz Enzyme Nomenclature


Accepted name:
cis-aconitate decarboxylase
Other names:
cis-aconitic decarboxylase
cis-aconitate carboxy-lyase
CAD1 (gene name)
IRG1 (gene name)
Systematic name:
cis-aconitate carboxy-lyase (itaconate-forming)



The enzyme has been characterized from the fungus Aspergillus terreus and from human macrophages. cf. EC, trans-aconitate decarboxylase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0047613
CAS Registry Number: 9025-01-8


  1. Bentley, R. and Thiessen, C.P.
    Biosynthesis of itaconic acid in Aspergillus terreus. III. The properties and reaction mechanism of cis-aconitic acid decarboxylase.
    J. Biol. Chem. 226: 703-720 (1957). [PMID: 13438855]
  2. Dwiarti, L., Yamane, K., Yamatani, H., Kahar, P., Okabe, M.
    Purification and characterization of cis-aconitic acid decarboxylase from Aspergillus terreus TN484-M1.
    J. Biosci. Bioeng. 94: 29-33 (2002). [PMID: 16233265]
  3. Kanamasa, S., Dwiarti, L., Okabe, M., Park, E. Y.
    Cloning and functional characterization of the cis-aconitic acid decarboxylase (CAD) gene from Aspergillus terreus.
    Appl. Microbiol. Biotechnol. 80: 223-229 (2008). [PMID: 18584171]
  4. Michelucci, A., Cordes, T., Ghelfi, J., Pailot, A., Reiling, N., Goldmann, O., Binz, T., Wegner, A., Tallam, A., Rausell, A., Buttini, M., Linster, C. L., Medina, E., Balling, R., Hiller, K.
    Immune-responsive gene 1 protein links metabolism to immunity by catalyzing itaconic acid production.
    Proc. Natl. Acad. Sci. U.S.A. 110: 7820-7825 (2013). [PMID: 23610393]

[EC created 1961, modified 2018]