EC - 2-haloacid dehalogenase (configuration-inverting)

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IntEnz Enzyme Nomenclature


Accepted name:
2-haloacid dehalogenase (configuration-inverting)
Other names:
2-haloalkanoic acid dehalogenase
2-haloalkanoid acid halidohydrolase
DL-2-haloacid dehalogenase
DL-2-haloacid dehalogenase (inversion of configuration)
DL-2-haloacid halidohydrolase (inversion of configuration)
Systematic name:
(R,S)-2-haloacid dehalogenase (configuration-inverting)



Dehalogenates both (S)- and (R)-2-haloalkanoic acids to the corresponding (R)- and (S)-hydroxyalkanoic acids, respectively, with inversion of configuration at C-2. The enzyme from Pseudomonas sp. 113 acts on 2-haloalkanoic acids whose carbon chain lengths are five or less. [See also EC, (S)-2-haloacid dehalogenase, EC, (R)-2-haloacid dehalogenase and EC, 2-haloacid dehalogenase (configuration-retaining)].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0033976 , GO:0018778


  1. Motosugi, K., Esahi, N. and Soda, K.
    Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase.
    Arch. Microbiol. 131 : 179-183 (1982). [PMID: 7103659]
  2. Motosugi, K., Esahi, N. and Soda, K.
    Purification and properties of a new enzyme, DL-2-haloacid dehalogenase, from Pseudomonas sp.
    J. Bacteriol. 150 : 522-527 (1982). [PMID: 7068529]
  3. Motosugi, K., Esahi, N. and Soda, K.
    Enzymatic preparation of D- and L-lactic acid from racemic 2-chloropropionic acid.
    Biotechnol. Bioeng. 26 : 805-806 (1984). [PMID: 18889931]
  4. Kurihara, T., Esaki, N. and Soda, K.
    Bacterial 2-haloacid dehalogenases: structures and reaction mechanisms.
    J. Mol. Catal., B Enzym. 10 : 57-65 (2000).
  5. Liu, J.-Q., Kurihara, T., Hasan, A.K.M.Q., Nardi-Dei, V., Koshikawa, H., Esaki, N. and Soda, K.
    Purification and characterization of thermostable and nonthermostable 2-haloacid dehalogenases with different stereospecificities from Pseudomonas sp. strain YL.
    Appl. Environ. Microbiol. 60 : 2389-2393 (1994). [PMID: 8074519]
  6. Cairns, S.S., Cornish, A. and Cooper, R.A.
    Cloning, sequencing and expression in Escherichia coli of two Rhizobium sp. genes encoding haloalkanoate dehalogenases of opposite stereospecificity.
    Eur. J. Biochem. 235 : 744-749 (1996). [PMID: 8654424]
  7. Leigh, J.A., Skinner, A.J. and Cooper, R.A.
    Partial purification, stereospecificity and stoichiometry of three dehalogenases from a Rhizobium species.
    FEMS Microbiol. Lett. 49 : 353-356 (1988).
  8. Weightman, A.J., Weightman, A.L. and Slater, J.H.
    Stereospecificity of 2-monochloropropionate dehalogenation by the two dehalogenases of Pseudomonas P3: evidence for two different dehalogenation mechanisms.
    J. Gen. Microbiol. 128 : 1755-1762 (1982). [PMID: 7142958]
  9. Soda, K., Kurihara, T., Liu, J.-Q., Nardi-Dei, V., Park, C., Miyagi, M., Tsunasawa, S. and Esaki, N.
    Bacterial 2-haloacid dehalogenases: Structures and catalytic properties.
    Pure Appl. Chem. 68 : 2097-2103 (1996).

[EC created 2003]