EC - 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase

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IntEnz Enzyme Nomenclature


Accepted name:
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
Other names:
tesD (gene name)
hsaD (gene name)
Systematic name:
4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase ( (2Z,4Z)-2-hydroxyhexa-2,4-dienoate-forming)



The enzyme is involved in the bacterial degradation of the steroid ring structure, and is involved in degradation of multiple steroids, such as testosterone [1], cholesterol [2], and sitosterol.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0102296


  1. Horinouchi, M., Hayashi, T., Koshino, H., Kurita, T., Kudo, T.
    Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441.
    Appl. Environ. Microbiol. 71: 5275-5281 (2005). [PMID: 16151114]
  2. Van der Geize, R., Yam, K., Heuser, T., Wilbrink, M. H., Hara, H., Anderton, M. C., Sim, E., Dijkhuizen, L., Davies, J. E., Mohn, W. W., Eltis, L. D.
    A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobacterium tuberculosis survival in macrophages.
    Proc. Natl. Acad. Sci. U.S.A. 104: 1947-1952 (2007). [PMID: 17264217]
  3. Lack, N., Lowe, E. D., Liu, J., Eltis, L. D., Noble, M. E., Sim, E., Westwood, I. M.
    Structure of HsaD, a steroid-degrading hydrolase, from Mycobacterium tuberculosis.
    Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64: 2-7 (2008). [PMID: 18097091]
  4. Lack, N. A., Yam, K. C., Lowe, E. D., Horsman, G. P., Owen, R. L., Sim, E., Eltis, L. D.
    Characterization of a carbon-carbon hydrolase from Mycobacterium tuberculosis involved in cholesterol metabolism.
    J. Biol. Chem. 285: 434-443 (2010). [PMID: 19875455]

[EC created 2012]