EC 3.6.1.59 - 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase

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IntEnz Enzyme Nomenclature
EC 3.6.1.59

Names

Accepted name:
5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase
Other names:
DcpS
m7GpppX pyrophosphatase
m7GpppN m7GMP phosphohydrolase
m7GpppX diphosphatase
Systematic name:
5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] N7-methylguanosine 5'-phosphate phosphohydrolase

Reactions

Comments:

The enzyme removes (decaps) the N7-methylguanosine 5-phosphate cap from an mRNA degraded to a maximal length of 10 nucleotides [3,6]. Decapping is an important process in the control of eukaryotic mRNA degradation. The enzyme functions to clear the cell of cap structure following decay of the RNA body [2]. The nematode enzyme can also decap triply methylated substrates, 5'-(N2,N2,N7-trimethyl 5'-triphosphoguanosine)-[mRNA] [4].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot:

References

  1. Malys, N., McCarthy, J. E.
    Dcs2, a novel stress-induced modulator of m7GpppX pyrophosphatase activity that locates to P bodies.
    J. Mol. Biol. 363: 370-382 (2006). [PMID: 16963086]
  2. Liu, S. W., Rajagopal, V., Patel, S. S., Kiledjian, M.
    Mechanistic and kinetic analysis of the DcpS scavenger decapping enzyme.
    J. Biol. Chem. 283: 16427-16436 (2008). [PMID: 18441014]
  3. Liu, H., Rodgers, N. D., Jiao, X., Kiledjian, M.
    The scavenger mRNA decapping enzyme DcpS is a member of the HIT family of pyrophosphatases.
    EMBO J. 21: 4699-4708 (2002). [PMID: 12198172]
  4. van Dijk, E., Le Hir, H., Seraphin, B.
    DcpS can act in the 5'-3' mRNA decay pathway in addition to the 3'-5' pathway.
    Proc. Natl. Acad. Sci. U.S.A. 100: 12081-12086 (2003). [PMID: 14523240]
  5. Chen, N., Walsh, M. A., Liu, Y., Parker, R., Song, H.
    Crystal structures of human DcpS in ligand-free and m7GDP-bound forms suggest a dynamic mechanism for scavenger mRNA decapping.
    J. Mol. Biol. 347: 707-718 (2005). [PMID: 15769464]
  6. Cohen, L. S., Mikhli, C., Friedman, C., Jankowska-Anyszka, M., Stepinski, J., Darzynkiewicz, E., Davis, R. E.
    Nematode m7GpppG and m3(2,2,7)GpppG decapping: activities in Ascaris embryos and characterization of C. elegans scavenger DcpS.
    RNA 10: 1609-1624 (2004). [PMID: 15383679]
  7. Wypijewska, A., Bojarska, E., Lukaszewicz, M., Stepinski, J., Jemielity, J., Davis, R. E., Darzynkiewicz, E.
    7-methylguanosine diphosphate (m(7)GDP) is not hydrolyzed but strongly bound by decapping scavenger (DcpS) enzymes and potently inhibits their activity.
    Biochemistry 51: 8003-8013 (2012). [PMID: 22985415]

[EC 3.6.1.59 created 2012, modified 2013]