EC 3.6.1.58 - 8-oxo-dGDP phosphatase
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IntEnz Enzyme Nomenclature
EC 3.6.1.58
Names
Accepted name:
8-oxo-dGDP phosphatase
Other
name:
NUDT5
Systematic name:
8-oxo-dGDP phosphohydrolase
Reactions
- (1) 8-oxo-dGDP + H2O = 8-oxo-dGMP + phosphate
- (2) 8-oxo-GDP + H2O = 8-oxo-GMP + phosphate
Comments:
The enzyme catalyses the hydrolysis of both 8-oxo-dGDP and 8-oxo-GDP thereby preventing translational errors caused by oxidative damage. The preferred in vivo substrate is not known. The enzyme does not degrade 8-oxo-dGTP and 8-oxo-GTP to the monophosphates (cf. EC 3.6.1.55, 8-oxo-dGTP diphosphatase) [1,2]. Ribonucleotide diphosphates and deoxyribonucleotide diphosphates are hydrolysed with broad specificity. The bifunctional enzyme NUDT5 also hydrolyses ADP-ribose to AMP and D-ribose 5-phosphate (cf. EC 3.6.1.13, ADP-ribose diphosphatase) [4]. The human enzyme NUDT18 also hydrolyses 8-oxo-dADP and 2-hydroxy-dADP, the latter at a slower rate [6].
Links to other databases
Gene Ontology:
GO:0044716
,
GO:0044715
References
-
Mammalian enzymes for preventing transcriptional errors caused by oxidative damage.Nucleic Acids Res. 33 : 3779-3784 (2005). [PMID: 16002790]
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A novel mechanism for preventing mutations caused by oxidation of guanine nucleotides.EMBO Rep. 4 : 479-483 (2003). [PMID: 12717453]
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NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity.DNA Repair (Amst.) 8 : 1250-1254 (2009). [PMID: 19699693]
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Cleavage of oxidized guanine nucleotide and ADP sugar by human NUDT5 protein.J. Biochem. 149 : 731-738 (2011). [PMID: 21389046]
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Crystal structures of human NUDT5 reveal insights into the structural basis of the substrate specificity.J. Mol. Biol. 364 : 1021-1033 (2006). [PMID: 17052728]
[EC 3.6.1.58 created 2012]