IntEnz

EC 3.6.1.58 - 8-oxo-dGDP phosphatase

IntEnz Enzyme Nomenclature
EC 3.6.1.58

Names

Reactions

• (1) 8-oxo-dGDP + H2O = 8-oxo-dGMP + phosphate
• (2) 8-oxo-GDP + H2O = 8-oxo-GMP + phosphate

Comments:

The enzyme catalyses the hydrolysis of both 8-oxo-dGDP and 8-oxo-GDP thereby preventing translational errors caused by oxidative damage. The preferred in vivo substrate is not known. The enzyme does not degrade 8-oxo-dGTP and 8-oxo-GTP to the monophosphates (cf. EC 3.6.1.55, 8-oxo-dGTP diphosphatase) [1,2]. Ribonucleotide diphosphates and deoxyribonucleotide diphosphates are hydrolysed with broad specificity. The bifunctional enzyme NUDT5 also hydrolyses ADP-ribose to AMP and D-ribose 5-phosphate (cf. EC 3.6.1.13, ADP-ribose diphosphatase) [4]. The human enzyme NUDT18 also hydrolyses 8-oxo-dADP and 2-hydroxy-dADP, the latter at a slower rate [6].

Links to other databases

References

1. Ishibashi, T., Hayakawa, H., Ito, R., Miyazawa, M., Yamagata, Y., Sekiguchi, M.
   Mammalian enzymes for preventing transcriptional errors caused by oxidative damage.
   Nucleic Acids Res. 33 : 3779-3784 (2005). [PMID: 16002790]
2. Ishibashi, T., Hayakawa, H., Sekiguchi, M.
   A novel mechanism for preventing mutations caused by oxidation of guanine nucleotides.
   EMBO Rep. 4 : 479-483 (2003). [PMID: 12717453]
3. Kamiya, H., Hori, M., Arimori, T., Sekiguchi, M., Yamagata, Y., Harashima, H.
   NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity.
   DNA Repair (Amst.) 8 : 1250-1254 (2009). [PMID: 19699693]
4. Ito, R., Sekiguchi, M., Setoyama, D., Nakatsu, Y., Yamagata, Y., Hayakawa, H.
   Cleavage of oxidized guanine nucleotide and ADP sugar by human NUDT5 protein.
   J. Biochem. 149 : 731-738 (2011). [PMID: 21389046]
5. Zha, M., Zhong, C., Peng, Y., Hu, H., Ding, J.
   Crystal structures of human NUDT5 reveal insights into the structural basis of the substrate specificity.
   J. Mol. Biol. 364 : 1021-1033 (2006). [PMID: 17052728]

[EC 3.6.1.58 created 2012]