EC 3.6.1.5 - Apyrase
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IntEnz Enzyme Nomenclature
EC 3.6.1.5
Names
Accepted name:
apyrase
Other
names:
ADPase
ATP diphosphohydrolase [ambiguous]
ATP-diphosphatase [ambiguous]
adenosine diphosphatase
ATP-diphosphohydrolase
ATP diphosphohydrolase [ambiguous]
ATP-diphosphatase [ambiguous]
adenosine diphosphatase
ATP-diphosphohydrolase
Systematic name:
nucleoside triphosphate phosphohydrolase (nucleoside monophosphoate-forming)
Reactions
- (1) a nucleoside 5'-triphosphate + 2 H2O = a nucleoside 5'-phosphate + 2 phosphate
- (1a) a nucleoside 5'-triphosphate + H2O = a nucleoside 5'-diphosphate + phosphate
- (1b) a nucleoside 5'-diphosphate + H2O = a nucleoside 5'-phosphate + phosphate
Cofactor
Comments:
Apyrases are active against both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyse NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates. They differ from ATPases, which specifically hydrolyse ATP, by hydrolysing both ATP and ADP. The eukaryotic enzymes requires Ca2+, but Mg2+ can substitute. Most of the ecto-ATPases that occur on the cell surface and hydrolyse extracellular nucleotides belong to this enzyme family.
Links to other databases
Protein domains and families:
PROSITE:PDOC00952
Gene Ontology:
GO:0102490
,
GO:0102489
,
GO:0102485
,
GO:0102486
,
GO:0102491
,
GO:0102488
,
GO:0102487
CAS Registry Number:
9000-95-7
References
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Apyrase, pyrophosphatase and metaphosphatase of Penicillium chrysogenum.Arch. Biochem. Biophys. 37 : 224-234 (1952). [PMID: 14953432]
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[Partial purification and properties of potato apyrase.]Bull. Soc. Chim. Biol. 45 : 573-594 (1963). [PMID: 13930517]
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Purification and partial characterization of a calmodulin-stimulated nucleoside triphosphatase from pea nuclei.J. Biol. Chem. 262 : 10689-10694 (1987). [PMID: 3038893]
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Purification and properties of human placental ATP diphosphohydrolase.Eur. J. Biochem. 234 : 66-74 (1995). [PMID: 8529670]
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CD39 is an ecto-(Ca2+,Mg2+)-apyrase.J. Biol. Chem. 271 : 9898-9901 (1996). [PMID: 8626624]
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YND1, a homologue of GDA1, encodes membrane-bound apyrase required for Golgi N- and O-glycosylation in Saccharomyces cerevisiae.J. Biol. Chem. 274 : 21450-21456 (1999). [PMID: 10409709]
-
Gene ytkD of Bacillus subtilis encodes an atypical nucleoside triphosphatase member of the Nudix hydrolase superfamily.J. Bacteriol. 186 : 8380-8384 (2004). [PMID: 15576788]
[EC 3.6.1.5 created 1961, modified 1976, modified 2000, modified 2013]