EC - NAD+ diphosphatase

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IntEnz Enzyme Nomenclature


Accepted name:
NAD+ diphosphatase
Other names:
NPY1 (gene name)
nudC (gene name)
NUDT7 (gene name)
nicotinamide adenine dinucleotide pyrophosphatase
NADP pyrophosphatase
NADH pyrophosphatase
NAD+ phosphohydrolase
Systematic name:
NAD(H) phosphohydrolase



This enzyme, described from plants, animals, and bacteria, can act on both reduced and oxidized forms of its substrate, although enzymes from different organisms have different preferences. Also acts on other dinucleotides, including NADP(H), FAD(H2), and the thionicotinamide analogues of NAD+ and NADP+.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00695
Structural data: CSA , EC2PDB
Gene Ontology: GO:0000210
CAS Registry Number: 37289-33-1
UniProtKB/Swiss-Prot: (122) [show] [UniProt]


  1. Kornberg, A. and Pricer, W.E.
    Nucleotide pyrophosphatase.
    J. Biol. Chem. 182 : 763-778 (1950).
  2. Jacobson, J.B. and Kaplan, N.O.
    A reduced pyridine nucleotide pyrophosphatase.
    J. Biol. Chem. 226 : 427-437 (1957). [PMID: 13428775]
  3. Swartz, M.N., Kaplan, N.O. and Lamborg, M.F.
    A heat-activated diphosphopyridine nucleotide pyrophosphatase from Proteus vulgaris.
    J. Biol. Chem. 232 : 1051-1063 (1958). [PMID: 13549486]
  4. Kumar, S. A., Rao, N. A., Vaidyanathan, C. S.
    Nucleotidases in plants. I. Partial purification and properties of the enzyme hydrolyzing flavine adenine dinucleotide from mung bean seedlings (Phaseolus radiatus).
    Arch. Biochem. Biophys. 111 : 646-652 (1965). [PMID: 5862212]
  5. Anderson, B.M. and Lang, C.A.
    Nicotinamide-adenine dinucleotide pyrophosphatase in the growing and aging mosquito.
    Biochem. J. 101 : 392-396 (1966). [PMID: 4381708]
  6. Nakajima, Y., Fukunaga, N., Sasaki, S. and Usami, S.
    Preparation and properties of NADP pyrophosphatase from Proteus vulgaris.
    Biochim. Biophys. Acta 293 : 242-255 (1973). [PMID: 4405504]
  7. Frick, D. N., Bessman, M. J.
    Cloning, purification, and properties of a novel NADH pyrophosphatase. Evidence for a nucleotide pyrophosphatase catalytic domain in MutT-like enzymes.
    J. Biol. Chem. 270 : 1529-1534 (1995). [PMID: 7829480]
  8. Xu, W., Dunn, C. A., Bessman, M. J.
    Cloning and characterization of the NADH pyrophosphatases from Caenorhabditis elegans and Saccharomyces cerevisiae, members of a Nudix hydrolase subfamily.
    Biochem. Biophys. Res. Commun. 273 : 753-758 (2000). [PMID: 10873676]
  9. Jambunathan, N., Mahalingam, R.
    Analysis of Arabidopsis growth factor gene 1 (GFG1) encoding a nudix hydrolase during oxidative signaling.
    Planta 224 : 1-11 (2006). [PMID: 16328543]

[EC created 1972]