IntEnz

EC 3.5.1.92 - Pantetheine hydrolase

IntEnz Enzyme Nomenclature
EC 3.5.1.92

Names

Reaction

• 13445 [IUBMB]
  (R)-pantetheine

  (R)-pantetheine

  Name origin: UniProt - CHECKED (C)

  CHEBI:16753

  Formula: C11H22N2O4S
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  +

  H₂O

  H2O

  Name origin: UniProt - CHECKED (C)

  CHEBI:15377

  Formula: H2O
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  =

  (R)-pantothenate

  (R)-pantothenate

  Name origin: UniProt - CHECKED (C)

  CHEBI:29032

  Formula: C9H16NO5
  Charge: -1

  ChEBI compound status: CHECKED (C)

  

  +

  cysteamine

  cysteamine

  Name origin: UniProt - CHECKED (C)

  CHEBI:58029

  Formula: C2H8NS
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

Comments:

The enzyme hydrolyses only one of the amide bonds of pantetheine. The substrate analogues phosphopantetheine and CoA are not substrates. The enzyme recycles pantothenate (vitamin B₅) and produces cysteamine, a potent anti-oxidant [5].

Links to other databases

References

1. Duprè, S. and Cavallini, D.
   Purification and properties of pantetheinase from horse kidney.
   Methods Enzymol. 62: 262-267 (1979). [PMID: 440106]
2. Duprè, S., Chiaraluce, R., Nardini, M., Cannella, C., Ricci, G. and Cavallini, D.
   Continuous spectrophotometric assay of pantetheinase activity.
   Anal. Biochem. 142: 175-181 (1985). [PMID: 6549111]
3. Maras, B., Barra, D., Duprè, S. and Pitari, G.
   Is pantetheinase the actual identity of mouse and human vanin-1 proteins?
   FEBS Lett. 461: 149-152 (1999). [PMID: 10567687]
4. Aurrand-Lions, M., Galland, F., Bazin, H., Zakharyev, V.M., Imhof, B.A. and Naquet, P.
   Vanin-1, a novel GPI-linked perivascular molecule involved in thymus homing.
   Immunity 5: 391-405 (1996). [PMID: 8934567]
5. Pitari, G., Malergue, F., Martin, F., Philippe, J.M., Massucci, M.T., Chabret, C., Maras, B., Dupre, S., Naquet, P. and Galland, F.
   Pantetheinase activity of membrane-bound Vanin-1: lack of free cysteamine in tissues of Vanin-1 deficient mice.
   FEBS Lett. 483: 149-154 (2000). [PMID: 11042271]
6. Martin, F., Malergue, F., Pitari, G., Philippe, J.M., Philips, S., Chabret, C., Granjeaud, S., Mattei, M.G., Mungall, A.J., Naquet, P. and Galland, F.
   Vanin genes are clustered (human 6q22-24 and mouse 10A2B1) and encode isoforms of pantetheinase ectoenzymes.
   Immunogenetics 53: 296-306 (2001). [PMID: 11491533]
7. Pace, H.C. and Brenner, C.
   The nitrilase superfamily: classification, structure and function.
   Genome Biol. 2: 0001.1-0001.9 (2001). [PMID: 11380987]

[EC 3.5.1.92 created 2005]