EC 18.104.22.168 - Phthalyl amidase
IntEnz Enzyme Nomenclature
18297 [IUBMB]a phtalamidea phtalamideName origin: UniProt - CHECKED (C)Formula: C8H5NO3R
Charge: -1ChEBI compound status: CHECKED (C)H2OH2OName origin: UniProt - CHECKED (C)Formula: H2O
Charge: 0ChEBI compound status: CHECKED (C)=a primary aminea primary amineName origin: UniProt - CHECKED (C)Formula: H3NR
Charge: 1ChEBI compound status: CHECKED (C)
In the entry, "phthalyl" is used to mean "2-carboxybenzoyl". The enzyme from Xanthobacter agilis hydrolyses phthalylated amino acids, peptides, β-lactams, aromatic and aliphatic amines. The substituent on nitrogen may be an alkyl group, but may also be complex, giving an amino acid or peptide derivative. Substitutions on the phthalyl ring include 6-F, 6-NH2, 3-OH, and a nitrogen in the aromatic ring ortho to the carboxy group attached to the amine. No cofactors are required.
Links to other databases
Discovery, purification, and properties of o-phthalyl amidase from Xanthobacter agilis.J. Mol. Catal., B Enzym. 2: 53-69 (1996).
o-Phthalyl amidase in the synthesis of Loracarbef: process development using this novel biocatalyst.Biotechnol. Lett. 18: 875-880 (1996).
Selective deprotection of phthalyl protected proteins.Tetrahedron Lett. 37: 7469-7472 (1996).
Phthalyl amidase of Xanthobacter agilis.US Patent no. 5,445,959, Aug. 29, (1995).
[EC 22.214.171.124 created 1999]