EC - Phthalyl amidase

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
phthalyl amidase
Systematic name:
phthalyl-amide amidohydrolase



In the entry, "phthalyl" is used to mean "2-carboxybenzoyl". The enzyme from Xanthobacter agilis hydrolyses phthalylated amino acids, peptides, β-lactams, aromatic and aliphatic amines. The substituent on nitrogen may be an alkyl group, but may also be complex, giving an amino acid or peptide derivative. Substitutions on the phthalyl ring include 6-F, 6-NH2, 3-OH, and a nitrogen in the aromatic ring ortho to the carboxy group attached to the amine. No cofactors are required.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0047418
CAS Registry Number: 169150-79-2


  1. Briggs, B.S., Kreuzman, A.J., Whitesitt, C., Yeh, W.K., Zmijewski, M.
    Discovery, purification, and properties of o-phthalyl amidase from Xanthobacter agilis.
    J. Mol. Catal., B Enzym. 2: 53-69 (1996).
  2. Black, T.D., Briggs, B.S., Evans, R., Muth, W.L., Vangala, S., Zmijewski, M.J.
    o-Phthalyl amidase in the synthesis of Loracarbef: process development using this novel biocatalyst.
    Biotechnol. Lett. 18: 875-880 (1996).
  3. Costello, C., Kreuzman, A., Zmijewski, M.
    Selective deprotection of phthalyl protected proteins.
    Tetrahedron Lett. 37: 7469-7472 (1996).
  4. Briggs, B.S., Zmijewski, M.J.
    Phthalyl amidase of Xanthobacter agilis.
    US Patent no. 5,445,959, Aug. 29, (1995).

[EC created 1999]