EC - Serralysin

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IntEnz Enzyme Nomenclature


Accepted name:
Other names:
Escherichia freundii proteinase
Pseudomonas aeruginosa alk. protease
Pseudomonas aeruginosa alkaline proteinase
Serratia marcescens extracellular proteinase
Serratia marcescens metalloprotease
Serratia marcescens metalloproteinase
Systematic name:




A 50 kDa extracellular endopeptidase from Pseudomonas aeruginosa [1,2,6], Escherichia freundii [3], Serratia marcescens [4,5,6] and Erwinia chrysanthemi [7]. There is broad specificity in cleavage of the insulin B chain, with some species variations. The pH optimum for digesting various proteins is about 9 - 10. In peptidase family M10 (interstitial collagenase family). Formerly included in EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , MEROPS , UniPathway
Protein domains and families: PROSITE:PDOC00129
Structural data: CSA , EC2PDB
CAS Registry Number: 70851-98-8
UniProtKB/Swiss-Prot: (12) [show] [UniProt]


  1. Morihara, K., Tsuzuki, H. and Oka, T.
    Comparison of the specificities of various neutral proteinases from microorganisms.
    Arch. Biochem. Biophys. 123: 572-588 (1968). [PMID: 4967801]
  2. Morihara, K., Tsuzuki, H. and Oka, T.
    On the specificity of Pseudomonas aeruginosa alkaline proteinase with synthetic peptides.
    Biochim. Biophys. Acta 309: 414-429 (1973). [PMID: 4199986]
  3. Nakajima, M., Mizusawa, K. and Yoshida, F.
    Purification and properties of an extracellular proteinase of psychrophilic Escherichia freundii.
    Eur. J. Biochem. 44: 87-96 (1974). [PMID: 4212288]
  4. Decedue, C.J., Broussard, E.A., II, Larson, A.D. and Braymer, H.D.
    Purification and characterization of the extracellular proteinase of Serratia marcescens.
    Biochim. Biophys. Acta 569: 293-301 (1979). [PMID: 383155]
  5. Doerr, M. and Traub, W.H.
    Purification and characterization of two Serratia marcescens proteases.
    Zentralbl. Bakteriol., Mikrobiol. Hyg. Ser. A 257: 6-19 (1984). [PMID: 6380155]
  6. Nakahama, K., Yoshimura, K., Marumoto, R., Kikuchi, M., Lee, I.S., Hase, T. and Matsubara, H.
    Cloning and sequencing of Serratia protease gene.
    Nucleic Acids Res. 14: 5843-5856 (1986). [PMID: 3016665]
  7. Dahler, G.S., Barras, F. and Keen, N.T.
    Cloning of genes encoding extracellular metalloproteases from Erwinia chrysanthemi EC16.
    J. Bacteriol. 172: 5803-5815 (1990). [PMID: 2211513]
  8. Okuda, K., Morihara, K., Atsumi, Y., Takeuchi, H., Kawamoto, S., Kawasaki, H., Suzuki, K. and Fukushima, J.
    Complete nucleotide sequence of the structural gene for alkaline proteinase from Pseudomonas aeruginosa IFO 3455.
    Infect. Immun. 58: 4083-4088 (1990). [PMID: 2123832]

[EC created 1972 as EC, part transferred 1992 to EC]