IntEnz

EC 3.4.23.51 - HycI peptidase

IntEnz Enzyme Nomenclature
EC 3.4.23.51

Names

Reaction

• This enzyme specifically removes a 32-amino acid peptide from the C-terminus of the precursor of the large subunit of hydrogenase 3 [UniProtKB: P16431] in Escherichia coli by cleavage at the C-terminal side of Arg537.

Comments:

The reaction requires nickel to be bound to the precursor of the large subunit of hydrogenase 3. The endopeptidase uses the metal in the large subunit of [NiFe]-hydrogenases as a recognition motif [1]. In peptidase family A31.

Links to other databases

References

1. Theodoratou, E., Paschos, A., Magalon, A., Fritsche, E., Huber, R., Bock, A.
   Nickel serves as a substrate recognition motif for the endopeptidase involved in hydrogenase maturation.
   Eur. J. Biochem. 267 : 1995-1999 (2000). [PMID: 10727938]
2. Yang, F., Hu, W., Xu, H., Li, C., Xia, B., Jin, C.
   Solution structure and backbone dynamics of an endopeptidase HycI from Escherichia coli: implications for mechanism of the [NiFe] hydrogenase maturation.
   J. Biol. Chem. 282 : 3856-3863 (2007). [PMID: 17150961]

[EC 3.4.23.51 created 2009]