EC - Omptin

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IntEnz Enzyme Nomenclature


Accepted name:
Other names:
protease VII
protease A
gene ompT proteins
ompT protease
protein a
Systematic name:



A product of the ompT gene of Escherichia coli, and associated with the outer membrane. Omptin shows a preference for cleavage between consecutive basic amino acids, but is capable of cleavage when P1' is a non-basic residue [5,7]. Belongs in peptidase peptidase family A26.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , MEROPS , UniPathway
Protein domains and families: PROSITE:PDOC00657
Structural data: CSA , EC2PDB
CAS Registry Number: 150770-86-8


  1. Grodberg, J., Lundrigan, M.D., Toledo, D.L., Mangel, W.F. and Dunn, J.J.
    Complete nucleotide sequence and deduced amino acid sequence of the ompT gene of Escherichia coli K-12.
    Nucleic Acids Res. 16 : 1209 (1988). [PMID: 3278297]
  2. Sugimura, K. and Nishihara, T.
    Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and ompT.
    J. Bacteriol. 170 : 5625-5632 (1988). [PMID: 3056908]
  3. Hanke, C., Hess, J., Schumacher, G. and Goebel, W.
    Processing by OmpT of fusion proteins carrying the HlyA transport signal during secretion by the Escherichia coli hemolysin transport system.
    Mol. Gen. Genet. 233 : 42-48 (1992). [PMID: 1603076]
  4. Dekker, N.
    In: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. (Eds.) Handbook of Proteolytic Enzymes , 2nd ed. , Elsevier , London , 2004 , 212-216
  5. Vandeputte-Rutten, L., Kramer, R.A., Kroon, J., Dekker, N., Egmond, M. R. and Gros, P.
    Crystal structure of the outer membrane protease OmpT from Escherichia coli suggests a novel catalytic site.
    EMBO J. 20 : 5033-5039 (2001). [PMID: 11566868]
  6. Kramer, R.A., Vandeputte-Rutten, L., de Roon, G.J., Gros, P., Dekker, N. and Egmond, M.R.
    Identification of essential acidic residues of outer membrane protease OmpT supports a novel active site.
    FEBS Lett. 505 : 426-430 (2001). [PMID: 11576541]
  7. McCarter, J.D., Stephens, D., Shoemaker, K., Rosenberg, S., Kirsch, J.F. and Georgiou, G.
    Substrate specificity of the Escherichia coli outer membrane protease OmpT.
    J. Bacteriol. 186 : 5919-5925 (2004). [PMID: 15317797]

[EC created 1993 as EC, transferred 2006 to EC]