EC - Prepilin peptidase

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IntEnz Enzyme Nomenclature


Accepted name:
prepilin peptidase
Systematic name:



Many species of bacteria carry pili on their cell surfaces. These are virulence determinants in pathogenic strains, and are assembled biosynthetically from type IV prepilin subunits. Before assembly, the prepilin molecules require proteolytic processing, which is done by the prepilin peptidase. Prepilin peptidase and its homologues play a central role not only in type IV pilus biogenesis but also in transport of macromolecules across cell membranes. Although both peptide-bond hydrolysis and N-methylation are catalysed by the same molecule, the methylation can be inhibited without affecting peptidase activity, and it is believed that the enzyme has two separate catalytic sites. Type example of peptidase family A24.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , MEROPS , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0008978
UniProtKB/Swiss-Prot: (23) [show] [UniProt]


  1. Lory, S. and Strom, M.S.
    Structure-function relationship of type-IV prepilin peptidase of Pseudomonas aeruginosa - A review.
    Gene 192: 117-121 (1997). [PMID: 9224881]
  2. LaPointe, C.F. and Taylor, R.K.
    The type 4 prepilin peptidases comprise a novel family of aspartic acid proteases.
    J. Biol. Chem. 275: 1502-1510 (2000). [PMID: 10625704]

[EC created 2001]