EC 3.4.23.32 - Scytalidopepsin B

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IntEnz Enzyme Nomenclature
EC 3.4.23.32

Names

Accepted name:
scytalidopepsin B
Other names:
Ganoderma lucidum aspartic proteinase
Ganoderma lucidum carboxyl proteinase
Scytalidium lignicolum aspartic proteinase B
Scytalidium aspartic proteinase B
SLB
Systematic name:

Reaction

Comments:

A second endopeptidase from Scytalidium lignicolum (see scytalidopepsin A) that is insensitive to pepstatin and methyl 2-diazoacetamidohexanoate. 1,2-Epoxy-3-(p-nitrophenoxy)propane reacts with Glu53, which replaces one of the aspartic residues at the active centre. One of the smallest aspartic endopeptidases active as the monomer, with Mr 22,000. Similarly inhibitor-resistant endopeptidases are found in the basidiomycetes Lentinus edodes [1] and Ganoderma lucidum [3], and in Polyporus tulipiferae [4], a second endopeptidase distinct from polyporopepsin, but these are of typical aspartic endopeptidase size, Mr about 36,000. Type example of peptidase family G1.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , MEROPS , UniPathway
Protein domains and families: PROSITE:PDOC00128
Structural data: CSA , EC2PDB
Gene Ontology: GO:0046560
CAS Registry Number: 104781-89-7
UniProtKB/Swiss-Prot:

References

  1. Terashita, T., Oda, K., Kono, M. and Murao, S.
    Streptomyces pepsin inhibitor-insensitive carboxyl proteinase from Lentinus edodes.
    Agric. Biol. Chem. 45: 1937-1943 (1981).
  2. Maita, T., Nagata, S., Matsuda, G., Maruta, S., Oda, K., Murao, S. and Tsuru, D.
    Complete amino acid sequence of Scytalidium lignicolum acid protease B.
    J. Biochem. (Tokyo) 95: 465-473 (1984).
  3. Terashita, T., Oda, K., Kono, M. and Murao, S.
    Streptomyces pepsin inhibitor-insensitive carboxyl proteinase from Ganoderma lucidum.
    Agric. Biol. Chem. 48: 1029-1035 (1984). [PMID: 6370989]
  4. Kobayashi, H., Kusakabe, I. and Murakami, K.
    Purification and characterization of a pepstatin-insensitive carboxyl proteinase from Polyporus tulipiferae (Irpex lacteus).
    Agric. Biol. Chem. 49: 2393-2397 (1985). [PMID: 6370989]
  5. Tsuru, D., Shimada, S., Maruta, S., Yoshimoto, T., Oda, K., Murao, S., Miyata, T. and Iwanaga, S.
    Isolation and amino acid sequence of a peptide containing an epoxide-reactive residue from the thermolysin-digest of Scytalidium lignicolum acid protease B.
    J. Biochem. (Tokyo) 99: 1537-1539 (1986). [PMID: 3519605]

[EC 3.4.23.32 created 1992]