EC - Stem bromelain

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IntEnz Enzyme Nomenclature


Accepted name:
stem bromelain
Systematic name:



The most abundant of the cysteine endopeptidases of the stem of the pineapple plant, Ananas comosus. Distinct from the bromelain found in the pineapple fruit (EC Scarcely inhibited by chicken cystatin and also very slowly inactivated by E-64. In peptidase family C1 (papain family). Formerly EC and included in

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , MEROPS , UniPathway
Protein domains and families: PROSITE:PDOC00126
Structural data: CSA , EC2PDB
CAS Registry Number: 37189-34-7


  1. Brocklehurst, K., Willenbrock, F. and Salih, E.
    Cysteine proteinases.
    In: Neuberger, A. and Brocklehurst, K. (Eds.) New Comprehensive Biochemistry vol. 16, Elsevier, Amsterdam, 1987, 39-158
  2. Rowan, A.D., Buttle, D.J. and Barrett, A.J.
    Ananain: a novel cysteine proteinase found in pineapple stem.
    Arch. Biochem. Biophys. 267: 262-270 (1988). [PMID: 3196029]
  3. Ritonja, A., Rowan, A.B., Buttle, D.J., Rawlings, N.D., Turk, V. and Barrett, A.J.
    Stem bromelain: amino acid sequence and implications for weak binding to cystatin.
    FEBS Lett. 247: 419-429 (1989). [PMID: 2714443]
  4. Rowan, A.D., Buttle, D.J. and Barrett, A J.
    The cysteine proteinases of the pineapple plant.
    Biochem. J. 266: 869-875 (1990). [PMID: 2327970]

[EC created 1965 as EC, transferred 1972 to EC, part transferred 1992 to EC]