EC 188.8.131.52 - Thermitase
IntEnz Enzyme Nomenclature
thermophilic Streptomyces serine proteinase
- Hydrolysis of proteins, including collagen.
A peptidase of family S8 (subtilisin family) from Thermoactinomyces vulgaris containing a single Cys, near the active site His, and inhibited by p-mercuribenzoate. The N-terminal extension of the polypeptide chain relative to subtilisin contributes to Ca2+-binding and the high thermostability. The amino acid composition and properties of the thermostable enzyme from Streptomyces rectus var. proteolyticus (formerly included in EC 184.108.40.206) are closely similar [1,2].
Links to other databases
Thermophilic Streptomyces alkaline proteinase.J. Biol. Chem. 247: 6978-6984 (1972). [PMID: 4711613]
Properties of two homologous alkaline proteases from Streptomyces rectus.J. Bacteriol. 123: 1109-1115 (1974). [PMID: 4373436]
Properties of thermitase, a thermostable serine protease from Thermoactinomyces vulgaris.Acta Biol. Med. Ger. 41: 89-102 (1982). [PMID: 7051706]
Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases.FEBS Lett. 183: 195-200 (1985).
Crystal structure of thermitase at 1.4 Å resolution.J. Mol. Biol. 214: 261-279 (1990). [PMID: 2196375]
[EC 220.127.116.11 created 1992]