EC - Thermitase

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IntEnz Enzyme Nomenclature


Accepted name:
Other names:
Thermoactinomyces vulgaris serine proteinase
thermophilic Streptomyces serine proteinase
Systematic name:



A peptidase of family S8 (subtilisin family) from Thermoactinomyces vulgaris containing a single Cys, near the active site His, and inhibited by p-mercuribenzoate. The N-terminal extension of the polypeptide chain relative to subtilisin contributes to Ca2+-binding and the high thermostability. The amino acid composition and properties of the thermostable enzyme from Streptomyces rectus var. proteolyticus (formerly included in EC are closely similar [1,2].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , MEROPS , UniPathway
Protein domains and families: PROSITE:PDOC00125
Structural data: CSA , EC2PDB
CAS Registry Number: 69772-87-8


  1. Mizusawa, K. and Yoshida, F.
    Thermophilic Streptomyces alkaline proteinase.
    J. Biol. Chem. 247: 6978-6984 (1972). [PMID: 4711613]
  2. Borgia, P. and Campbell, L.
    Properties of two homologous alkaline proteases from Streptomyces rectus.
    J. Bacteriol. 123: 1109-1115 (1974). [PMID: 4373436]
  3. Kleine, R.
    Properties of thermitase, a thermostable serine protease from Thermoactinomyces vulgaris.
    Acta Biol. Med. Ger. 41: 89-102 (1982). [PMID: 7051706]
  4. Meloun, B., Baudyš, M., Kostka, V., Hausdorf, G., Frömmel, C. and Höhne, W.E.
    Complete primary structure of thermitase from Thermoactinomyces vulgaris and its structural features related to the subtilisin-type proteinases.
    FEBS Lett. 183: 195-200 (1985).
  5. Teplyakov, A.V., Kuranova, I.P., Harutyunyan, E.H., Vainshtein, B.K., Frömmel, C., Höhne, W.E. and Wilson, K.S.
    Crystal structure of thermitase at 1.4 Å resolution.
    J. Mol. Biol. 214: 261-279 (1990). [PMID: 2196375]

[EC created 1992]