EC - Tissue kallikrein

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IntEnz Enzyme Nomenclature


Accepted name:
tissue kallikrein
Other names:
dilminal D
glandular kallikrein
kidney kallikrein
onokrein P
pancreatic kallikrein
salivary kallikrein
submandibular kallikrein
submaxillary kallikrein
urinary kallikrein
Systematic name:



Formed from tissue prokallikrein by activation with trypsin. In peptidase family S1 (trypsin family). Formerly EC and included in EC A large number of tissue kallikrein-related sequences have been reported for rats [16] and mice [7], though fewer seem to exist in other mammals. The few that have been isolated and tested on substrates include mouse γ-renin (EC, submandibular proteinase A (formerly EC [2, 15], epidermal growth-factor-binding protein, nerve growth factor γ-subunit, rat tonin [3, 4, 9], submaxillary proteinases A and B [10], T-kininogenase [18], kallikreins k7 and k8 [17] and human prostate-specific antigen (γ-seminoprotein) [6].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , MEROPS , UniPathway
Protein domains and families: PROSITE:PDOC00124
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004293
CAS Registry Number: 9001-01-8 (not distinguished from EC and EC
UniProtKB/Swiss-Prot: (29) [show] [UniProt]


  1. Fiedler, F., Fink, E., Tschesche, H. and Fritz, H.
    Porcine glandular kallikreins.
    Methods Enzymol. 80C: 493-532 (1981). [PMID: 7043199]
  2. Anundi, H., Ronne, H., Peterson, P.A. and Rask, L.
    Partial amino-acid sequence of the epidermal growth-factor-binding protein.
    Eur. J. Biochem. 129: 365-371 (1982). [PMID: 6295764]
  3. Pesquero, J.L., Boschcov, P., Oliveira, M.C.F. and Paiva, A.C.M.
    Effect of substrate size on tonin activity.
    Biochem. Biophys. Res. Commun. 108: 1441-1446 (1982). [PMID: 6295383]
  4. Gutkowska, J., Corvol, P., Figueiredo, A.F.S., Inagami, T., Bouhnik, J. and Genest, J.
    Kinetic studies of rat renin and tonin on purified rat angiotensinogen.
    Can. J. Biochem. Cell Biol. 62: 136-142 (1984). [PMID: 6043136]
  5. Kato, H., Enjyoji, K., Miyata, T., Hayashi, I., Oh-Ishi, S. and Iwanaga, S.
    Demonstration of arginyl-bradykinin moiety in rat HMW kininogen: direct evidence for liberation of bradykinin by rat glandular kallikreins.
    Biochem. Biophys. Res. Commun. 127: 289-295 (1985). [PMID: 3844939]
  6. Akiyama, K., Nakamura, T., Iwanaga, S. and Hara, M.
    The chymotrypsin-like activity of human prostate-specific antigen, γ-seminoprotein.
    FEBS Lett. 225: 168-172 (1987). [PMID: 3691800]
  7. Evans, B.A., Drinkwater, C.C. and Richards, R.I.
    Mouse glandular kallikrein genes. Structure and partial sequence analysis of the kallikrein gene locus.
    J. Biol. Chem. 262: 8027-8034 (1987). [PMID: 3036794]
  8. Fiedler, F.
    Effects of secondary interactions on the kinetics of peptide and peptide ester hydrolysis by tissue kallikrein and trypsin.
    Eur. J. Biochem. 163: 303-312 (1987). [PMID: 3643848]
  9. Fujinaga, M. and James, M.N.G.
    Rat submaxillary gland serine protease, tonin. Structure solution and refinement at 1.8 Å resolution.
    J. Mol. Biol. 195: 373-396 (1987). [PMID: 2821276]
  10. Kato, H., Nakanishi, E., Enjyoji, K., Hayashi, I., Oh-ishi, S. and Iwanaga, S.
    Characterization of serine proteinases isolated from rat submaxillary gland: with special reference to the degradation of rat kininogens by these enzymes.
    J. Biochem. (Tokyo) 102: 1389-1404 (1987). [PMID: 3844939]
  11. Bailey, G.S.
    Rat pancreas kallikrein.
    Methods Enzymol. 163: 115-128 (1989). [PMID: 3237072]
  12. Blaber, M., Isackson, P.J., Marsters, J.C., Jr, Burnier, J.P. and Bradshaw, R.A.
    Substrate specificities of growth factor associated kallikreins of the mouse submandibular gland.
    Biochemistry 28: 7813-7819 (1988). [PMID: 2611215]
  13. Chao, J. and Chao, L.
    Rat urinary kallikrein.
    Methods Enzymol. 163: 128-143 (1988). [PMID: 3070295]
  14. Geiger, R. and Miska, W.
    Human tissue kallikrein.
    Methods Enzymol. 163: 102-115 (1988). [PMID: 3237071]
  15. Bertrand, R., Derancourt, J. and Kassab, R.
    Selective cleavage at lysine of the 50 kDa-20 kDa connector loop segment of skeletal myosin S-1 by endoproteinase Arg-C.
    FEBS Lett. 246: 171-176 (1989). [PMID: 2523317]
  16. Wines, D.R., Brady, J.M., Pritchett, D.B., Roberts, J.L. and MacDonald, R.J.
    Organization and expression of the rat kallikrein gene family.
    J. Biol. Chem. 264: 7653-7662 (1989). [PMID: 2708383]
  17. Elmoujahed, A., Gutman, N., Brillard, M. and Gauthier, F.
    Substrate specificity of two kallikrein family gene products isolated from the rat submaxillary gland.
    FEBS Lett. 265: 137-140 (1990). [PMID: 2194829]
  18. Xiong, W., Chen, L.-M. and Chao, J.
    Purification and characterization of a kallikrein-like T-kininogenase.
    J. Biol. Chem. 265: 2822-2827 (1990). [PMID: 2303430]

[EC created 1965 as EC, transferred 1972 to, part transferred 1981 to EC]