EC - Cucumisin

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IntEnz Enzyme Nomenclature


Accepted name:
Systematic name:



From the sarcocarp of the musk melon (Cucumis melo). In peptidase family S8 (subtilisin family). Other endopeptidases from plants, which are less well characterized but presumably of serine-type, include euphorbain from Euphorbia cerifera ([6], formerly EC, solanain from horse-nettle Solanum elaeagnifolium ([1], formerly EC, hurain from Hura crepitans ([2], formerly EC and tabernamontanain from Tabernamontana grandiflora ([3], formerly EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , MEROPS , UniPathway
Protein domains and families: PROSITE:PDOC00125
Structural data: CSA , EC2PDB
CAS Registry Number: 82062-89-3


  1. Greenberg, D.M. and Winnick, T.
    Plant proteases. I. Activation-inhibition reactions.
    J. Biol. Chem. 135 : 761-773 (1940).
  2. Jaffé, W.G.
    Hurain, a new plant protease from Hura crepitans.
    J. Biol. Chem. 149 : 1-7 (1943).
  3. Jaffé, W.G.
    A new vegetable proteolytic enzyme of the papain class.
    Rev. Brasil Biol. 3 : 149-157 (1943).
  4. Kaneda, M. and Toninaga, N.
    Isolation and characterization of a proteinase from the sarcocarp of melon fruit.
    J. Biochem. (Tokyo) 78 : 1287-1296 (1975). [PMID: 5423]
  5. Kaneda, M., Ohmine, H., Yonezawa, H. and Tominaga, N.
    Amino acid sequence around the reactive site of cucumisin from melon fruit.
    J. Biochem. (Tokyo) 95 : 825-829 (1984). [PMID: 6427203]
  6. Lynn, K.R. and Clevette-Radford, N.A.
    Two proteases from the latex of Elaeophorbia drupifera.
    Phytochemistry 24 : 2843-2845 (1985).
  7. Kaneda, N., Minematsu, Y., Powers, J.C. and Tominaga, N.
    Specificity of cucumisin in hydrolysis of peptide thiobenzyl esters.
    Agric. Biol. Chem. 50 : 1075-1076 (1986).

[EC created 1978]