EC - Pestivirus NS3 polyprotein peptidase

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
pestivirus NS3 polyprotein peptidase
Other names:
border disease virus NS3 endopeptidase
BDV NS3 endopeptidase
bovine viral diarrhea virus NS3 endopeptidase
BVDV NS3 endopeptidase
classical swine fever virus NS3 endopeptidase
CSFV NS3 endopeptidase
Systematic name:



The polyprotein of noncytopathogenic pestiviruses is cleaved co- and post-translationally into at least 11 proteins (Npro, C, Erns, E1, E2, p7, NS2-3, NS4A, NS4B, NS5A, and NS5B) [2]. The genomes of cytopathogenic pestivirus strains express at least one additional protein, called NS3 (p80) [2]. This enzyme, which resides in the N-terminal region of NS3 (nonstructural protein 3), is essential for generation of its own C-terminus and for processing of the downstream cleavage sites, leading to the release of the pestivirus nonstructural proteins NS4A, NS4B, NS5A and NS5B [1,2]. Belongs in peptidase family S31.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , MEROPS , UniPathway
Structural data: CSA , EC2PDB


  1. Wiskerchen, M. and Collett, M.S.
    Pestivirus gene expression: protein p80 of bovine viral diarrhea virus is a proteinase involved in polyprotein processing.
    Virology 184 : 341-350 (1991). [PMID: 1651596]
  2. Tautz, N., Elbers, K., Stoll, D., Meyers, G. and Thiel, H.-J.
    Serine protease of pestiviruses: determination of cleavage sites.
    J. Virol. 71 : 5415-5422 (1997). [PMID: 9188613]
  3. Xu, J., Mendez, E., Caron, P.R., Lin, C., Murcko, M.A., Collett, M.S. and Rice, C.M.
    Bovine viral diarrhea virus NS3 serine proteinase: polyprotein cleavage sites, cofactor requirements, and molecular model of an enzyme essential for pestivirus replication.
    J. Virol. 71 : 5312-5322 (1997). [PMID: 9188600]
  4. Tautz, N., Kaiser, A. and Thiel, H.-J.
    NS3 serine protease of bovine viral diarrhea virus: characterization of active site residues, NS4A cofactor domain, and protease-cofactor interactions.
    Virology 273 : 351-363 (2000). [PMID: 10915606]

[EC created 2006]