EC 22.214.171.124 - Sedolisin
IntEnz Enzyme Nomenclature
- Hydrolysis of the B chain of insulin at -Glu13┼Ala-, -Leu15┼Tyr- and -Phe25┼Tyr-, and angiotensin I at -Tyr4┼Ile-. A good synthetic substrate is Lys-Pro-Ile-Glu-Phe┼Phe(NO2)-Arg-Leu.
An enzyme secreted by Pseudomonas sp. No. 101. Optimum pH is 4. It is distinguished from EC 126.96.36.199, xanthomonalisin, by its insensitivity to EPNP and from EC 188.8.131.52, scytalidopepsin B, by this property and by its unrelated amino-acid sequence. Inhibited by tyrostatin, a peptide aldehyde . Type example of peptidase family S53.
Links to other databases
Purification and properties of a pepstatin-insensitive carboxyl proteinase from a Gram-negative bacterium.Biochim. Biophys. Acta 923: 463-469 (1987). [PMID: 3548827]
Substrate specificity and kinetic properties of pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. No. 101.Biochim. Biophys. Acta 1120: 208-214 (1992). [PMID: 1562589]
Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes.Nat. Struct. Biol. 8: 442-446 (2001). [PMID: 11323721]
Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases.Acta Biochim. Pol. 50: 81-102 (2003). [PMID: 12673349]
[EC 184.108.40.206 created 1995 as EC 220.127.116.11, transferred 2001 to EC 18.104.22.168, modified 2003]