EC - Glutamate carboxypeptidase

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IntEnz Enzyme Nomenclature


Accepted name:
glutamate carboxypeptidase
Other names:
N-pteroyl-L-glutamate hydrolase
carboxypeptidase G
carboxypeptidase G1
carboxypeptidase G2
glutamyl carboxypeptidase
Systematic name:




A zinc enzyme produced by pseudomonads, Flavobacterium sp. and Acinetobacter sp. Its ability to hydrolyse pteroyl-L-glutamate (folic acid) has led to its use as a folate-depleting, antitumour agent. Type example of peptidase family M20.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , MEROPS , UniPathway
Protein domains and families: PROSITE:PDOC00613
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004180
CAS Registry Number: 9074-87-7


  1. Goldman, P. and Levy, C.C.
    Carboxypeptidase G: purification and properties.
    Proc. Natl. Acad. Sci. USA 58 : 1299-1306 (1967). [PMID: 5237864]
  2. McCullogh, J.L., Chabner, B.A. and Bertino, J.R.
    Purification and properties of carboxypeptidase G1.
    J. Biol. Chem. 246 : 7207-7213 (1971). [PMID: 5129727]
  3. Albrecht, A.M., Boldizar, E. and Hutchinson, D.J.
    Carboxypeptidase displaying differential velocity in hydrolysis of methotrexate, 5-methyltetrahydrofolic acid, and leucovorin.
    J. Bacteriol. 134 : 506-513 (1978). [PMID: 26657]
  4. Sherwood, R.F., Melton, R.G. and Alwan, S.A.
    Purification and properties of carboxypeptidase G2 from Pseudomonas sp. strain RS-16.
    Eur. J. Biochem. 148 : 447-453 (1985). [PMID: 3838935]

[EC created 1992]