EC - Aminopeptidase S

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IntEnz Enzyme Nomenclature


Accepted name:
aminopeptidase S
Other names:
Mername-AA022 peptidase
aminopeptidase (Streptomyces griseus)
Streptomyces griseus aminopeptidase
S. griseus AP
double-zinc aminopeptidase
Systematic name:




Aminopeptidases are associated with many biological functions, including protein maturation, protein degradation, cell-cycle control and hormone-level regulation [3,4]. This enzyme contains two zinc molecules in its active site and is activated by Ca2+ [4]. In the presence of Ca2+, the best substrates are Leu-Phe, Leu-Ser, Leu-pNA (aminoacyl-p-nitroanilide), Phe-Phe-Phe and Phe-Phe [3]. Peptides with proline in the P1' position are not substrates [3]. Belongs in peptidase family M28.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. Spungin, A. and Blumberg, S.
    Streptomyces griseus aminopeptidase is a calcium-activated zinc metalloprotein. Purification and properties of the enzyme.
    Eur. J. Biochem. 183: 471-477 (1989). [PMID: 2503378]
  2. Ben-Meir, D., Spungin, A., Ashkenazi, R. and Blumberg, S.
    Specificity of Streptomyces griseus aminopeptidase and modulation of activity by divalent metal ion binding and substitution.
    Eur. J. Biochem. 212: 107-112 (1993). [PMID: 8444149]
  3. Arima, J., Uesugi, Y., Iwabuchi, M. and Hatanaka, T.
    Study on peptide hydrolysis by aminopeptidases from Streptomyces griseus, Streptomyces septatus and Aeromonas proteolytica.
    Appl. Microbiol. Biotechnol. 70: 541-547 (2006). [PMID: 16080009]
  4. Fundoiano-Hershcovitz, Y., Rabinovitch, L., Langut, Y., Reiland, V., Shoham, G. and Shoham, Y.
    Identification of the catalytic residues in the double-zinc aminopeptidase from Streptomyces griseus.
    FEBS Lett. 571: 192-196 (2004). [PMID: 15280041]
  5. Gilboa, R., Greenblatt, H.M., Perach, M., Spungin-Bialik, A., Lessel, U., Wohlfahrt, G., Schomburg, D., Blumberg, S. and Shoham, G.
    Interactions of Streptomyces griseus aminopeptidase with a methionine product analogue: a structural study at 1.53 Å resolution.
    Acta Crystallogr. D Biol. Crystallogr. 56: 551-558 (2000). [PMID: 10771423]

[EC created 2008]