EC - Limonene-1,2-epoxide hydrolase

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IntEnz Enzyme Nomenclature


Accepted name:
limonene-1,2-epoxide hydrolase
Other name:
limonene oxide hydrolase
Systematic name:
1,2-epoxymenth-8-ene hydrolase



Involved in the monoterpene degradation pathway of the actinomycete Rhodococcus erythropolis. Enzyme hydrolyses several alicyclic and 1-methyl-substituted epoxides, such as 1-methylcyclohexene oxide, indene oxide and cyclohexene oxide. It differs from the previously described epoxide hydrolases [EC (trans-epoxysuccinate hydrolase), EC (leukotriene-A4 hydrolase), EC (hepoxilin-epoxide hydrolase), EC (microsomal epoxide hydrolase) and EC (soluble epoxide hydrolase)] as it is not inhibited by 2-bromo-4'-nitroacetophenone, diethyl pyrocarbonate, 4-fluorochalcone oxide or 1,10-phenanthroline.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UM-BBD , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0018744
CAS Registry Number: 216503-88-7


  1. van der Werf, M.J., Overkamp, K.M. and de Bont, J.A.M.
    Limonene-1,2-epoxide hydrolase from Rhodococcus erythropolis DCL14 belongs to a novel class of epoxide hydrolases.
    J. Bacteriol. 180 : 5052-5057 (1998). [PMID: 9748436]
  2. Barbirato, F., Verdoes, J.C., de Bont, J.A.M. and van der Werf, M.J.
    The Rhodococcus erythropolis DCL14 limonene-1,2-epoxide hydrolase gene encodes an enzyme belonging to a novel class of epoxide hydrolases.
    FEBS Lett. 438 : 293-296 (1998). [PMID: 9827564]
  3. van der Werf, M.J., Swarts, H.J. and de Bont, J.A.M.
    Rhodococcus erythropolis DCL14 contains a novel degradation pathway for limonene.
    Appl. Environ. Microbiol. 65 : 2092-2102 (1999). [PMID: 10224006]

[EC created 2001]