EC 3.2.2.31 - Adenine glycosylase

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IntEnz Enzyme Nomenclature
EC 3.2.2.31

Names

Accepted name:
adenine glycosylase
Other names:
mutY (gene name)
A/G-specific adenine glycosylase
Systematic name:
adenine-DNA deoxyribohydrolase (adenine-releasing)

Reaction

Comments:

The enzyme serves as a mismatch repair enzyme that works to correct 7,8-dihydro-8-oxoguanine:adenine mispairs that arise in DNA when error-prone synthesis occurs past 7,8-dihydro-8-oxoguanine (GO) lesions in DNA. The enzyme excises the adenine of the mispair, producing an apurinic site sensitive to AP endonuclease activity. After removing the undamaged adenine the enzyme remains bound to the site to prevent EC 3.2.2.23 (MutM) from removing the GO lesion, which could lead to a double strand break. In vitro the enzyme is also active with adenine:guanine, adenine:cytosine, and adenine:7,8-dihydro-8-oxoadenine (AO) mispairs, removing the adenine in all cases.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot: (17) [show] [UniProt]

References

  1. Au, K. G., Clark, S., Miller, J. H., Modrich, P.
    Escherichia coli mutY gene encodes an adenine glycosylase active on G-A mispairs.
    Proc. Natl. Acad. Sci. U.S.A. 86: 8877-8881 (1989). [PMID: 2682664]
  2. Michaels, M. L., Tchou, J., Grollman, A. P., Miller, J. H.
    A repair system for 8-oxo-7,8-dihydrodeoxyguanine.
    Biochemistry 31: 10964-10968 (1992). [PMID: 1445834]
  3. Lee, S., Verdine, G. L.
    Atomic substitution reveals the structural basis for substrate adenine recognition and removal by adenine DNA glycosylase.
    Proc. Natl. Acad. Sci. U.S.A. 106: 18497-18502 (2009). [PMID: 19841264]

[EC 3.2.2.31 created 2018]