EC - Aminodeoxyfutalosine nucleosidase

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
aminodeoxyfutalosine nucleosidase
Other names:
AFL nucleosidase
aminofutalosine nucleosidase
methylthioadenosine nucleosidase
Systematic name:
6-amino-6-deoxyfutalosine ribohydrolase



The enzyme, found in several bacterial species, catalyses a step in a modified futalosine pathway for menaquinone biosynthesis. While the enzyme from some organisms also has the activity of EC, adenosylhomocysteine nucleosidase, the enzyme from Chlamydia trachomatis is specific for 6-amino-6-deoxyfutalosine [7].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0102246


  1. Hiratsuka, T., Furihata, K., Ishikawa, J., Yamashita, H., Itoh, N., Seto, H., Dairi, T.
    An alternative menaquinone biosynthetic pathway operating in microorganisms.
    Science 321: 1670-1673 (2008). [PMID: 18801996]
  2. Li, X., Apel, D., Gaynor, E. C., Tanner, M. E.
    5'-methylthioadenosine nucleosidase is implicated in playing a key role in a modified futalosine pathway for menaquinone biosynthesis in Campylobacter jejuni.
    J. Biol. Chem. 286: 19392-19398 (2011). [PMID: 21489995]
  3. Arakawa, C., Kuratsu, M., Furihata, K., Hiratsuka, T., Itoh, N., Seto, H., Dairi, T.
    Diversity of the early step of the futalosine pathway.
    Antimicrob. Agents Chemother. 55: 913-916 (2011). [PMID: 21098241]
  4. Wang, S., Haapalainen, A. M., Yan, F., Du, Q., Tyler, P. C., Evans, G. B., Rinaldo-Matthis, A., Brown, R. L., Norris, G. E., Almo, S. C., Schramm, V. L.
    A picomolar transition state analogue inhibitor of MTAN as a specific antibiotic for Helicobacter pylori.
    Biochemistry 51: 6892-6894 (2012). [PMID: 22891633]
  5. Mishra, V., Ronning, D. R.
    Crystal structures of the Helicobacter pylori MTAN enzyme reveal specific interactions between S-adenosylhomocysteine and the 5'-alkylthio binding subsite.
    Biochemistry 51: 9763-9772 (2012). [PMID: 23148563]
  6. Kim, R. Q., Offen, W. A., Davies, G. J., Stubbs, K. A.
    Structural enzymology of Helicobacter pylori methylthioadenosine nucleosidase in the futalosine pathway.
    Acta Crystallogr. D Biol. Crystallogr. 70: 177-185 (2014). [PMID: 24419390]
  7. Barta, M. L., Thomas, K., Yuan, H., Lovell, S., Battaile, K. P., Schramm, V. L., Hefty, P. S.
    Structural and biochemical characterization of Chlamydia trachomatis hypothetical protein CT263 supports that menaquinone synthesis occurs through the futalosine pathway.
    J. Biol. Chem. 289: 32214-32229 (2014). [PMID: 25253688]

[EC created 2014]