EC 3.2.2.1 - Purine nucleosidase

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IntEnz Enzyme Nomenclature
EC 3.2.2.1

Names

Accepted name:
purine nucleosidase
Other names:
N-ribosyl purine ribohydrolase
nucleosidase [misleading]
nucleosidase g
nucleoside hydrolase [misleading]
purine β-ribosidase
purine nucleoside hydrolase
purine ribonucleosidase
ribonucleoside hydrolase [misleading]
N-D-ribosylpurine ribohydrolase
inosine-adenosine-guanosine preferring nucleoside hydrolase
purine-specific nucleoside N-ribohydrolase
IAG-nucleoside hydrolase
IAG-NH
Systematic name:
purine-nucleoside ribohydrolase

Reaction

Comments:

The enzyme from the bacterium Ochrobactrum anthropi specifically catalyses the irreversible N-riboside hydrolysis of purine nucleosides. Pyrimidine nucleosides, purine and pyrimidine nucleotides, NAD+, NADP+ and nicotinaminde mononucleotide are not substrates [6].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00960
Structural data: CSA , EC2PDB
Gene Ontology: GO:0008477
CAS Registry Number: 9025-44-9

References

  1. Heppel, L.A. and Hilmoe, R.J.
    Phosphorolysis and hydrolysis of purine ribosides by enzymes from yeast.
    J. Biol. Chem. 198: 683-694 (1952). [PMID: 12999785]
  2. Kalckar, H.M.
    Biosynthetic aspects of nucleosides and nucleic acids.
    Pubbl. Staz. Zool. (Napoli) 23: 87-103 (1951).
  3. Takagi, Y. and Horecker, B.L.
    Purification and properties of a bacterial riboside hydrolyase.
    J. Biol. Chem. 225: 77-86 (1956). [PMID: 13416219]
  4. Tarr, H.L.A.
    Fish muscle riboside hydrolases.
    Biochem. J. 59: 386-391 (1955). [PMID: 14363106]
  5. Parkin, D.W.
    Purine-specific nucleoside N-ribohydrolase from Trypanosoma brucei brucei. Purification, specificity, and kinetic mechanism.
    J. Biol. Chem. 271: 21713-21719 (1996). [PMID: 8702965]
  6. Ogawa, J., Takeda, S., Xie, S.-X., Hatanaka, H., Ashikari, T., Amachi, T. and Shimizu, S.
    Purification, characterization, and gene cloning of purine nucleosidase from Ochrobactrum anthropi.
    Appl. Environ. Microbiol. 67: 1783-1787 (2001). [PMID: 11282633]
  7. Versées, W., Decanniere, K., Van Holsbeke, E., Devroede, N. and Steyaert, J.
    Enzyme-substrate interactions in the purine-specific nucleoside hydrolase from Trypanosoma vivax.
    J. Biol. Chem. 277: 15938-15946 (2002). [PMID: 11854281]
  8. Mazumder-Shivakumar, D. and Bruice, T.C.
    Computational study of IAG-nucleoside hydrolase: determination of the preferred ground state conformation and the role of active site residues.
    Biochemistry 44: 7805-7817 (2005). [PMID: 15909995]

[EC 3.2.2.1 created 1961, modified 2006, modified 2011]