EC 3.2.2.1 - Purine nucleosidase
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IntEnz Enzyme Nomenclature
EC 3.2.2.1
Names
Accepted name:
purine nucleosidase
Other
names:
N-ribosyl purine ribohydrolase
nucleosidase [misleading]
nucleosidase g
nucleoside hydrolase [misleading]
purine β-ribosidase
purine nucleoside hydrolase
purine ribonucleosidase
ribonucleoside hydrolase [misleading]
N-D-ribosylpurine ribohydrolase
inosine-adenosine-guanosine preferring nucleoside hydrolase
purine-specific nucleoside N-ribohydrolase
IAG-nucleoside hydrolase
IAG-NH
nucleosidase [misleading]
nucleosidase g
nucleoside hydrolase [misleading]
purine β-ribosidase
purine nucleoside hydrolase
purine ribonucleosidase
ribonucleoside hydrolase [misleading]
N-D-ribosylpurine ribohydrolase
inosine-adenosine-guanosine preferring nucleoside hydrolase
purine-specific nucleoside N-ribohydrolase
IAG-nucleoside hydrolase
IAG-NH
Systematic name:
purine-nucleoside ribohydrolase
Reaction
- a purine nucleoside + H2O = D-ribose + a purine base
Comments:
The enzyme from the bacterium Ochrobactrum anthropi specifically catalyses the irreversible N-riboside hydrolysis of purine nucleosides. Pyrimidine nucleosides, purine and pyrimidine nucleotides, NAD+, NADP+ and nicotinaminde mononucleotide are not substrates [6].
Links to other databases
Protein domains and families:
PROSITE:PDOC00960
Gene Ontology:
GO:0008477
CAS Registry Number:
9025-44-9
UniProtKB/Swiss-Prot:
RIH_DICDI
References
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Phosphorolysis and hydrolysis of purine ribosides by enzymes from yeast.J. Biol. Chem. 198 : 683-694 (1952). [PMID: 12999785]
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Biosynthetic aspects of nucleosides and nucleic acids.Pubbl. Staz. Zool. (Napoli) 23 : 87-103 (1951).
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Purification and properties of a bacterial riboside hydrolyase.J. Biol. Chem. 225 : 77-86 (1956). [PMID: 13416219]
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Fish muscle riboside hydrolases.Biochem. J. 59 : 386-391 (1955). [PMID: 14363106]
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Purine-specific nucleoside N-ribohydrolase from Trypanosoma brucei brucei. Purification, specificity, and kinetic mechanism.J. Biol. Chem. 271 : 21713-21719 (1996). [PMID: 8702965]
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Purification, characterization, and gene cloning of purine nucleosidase from Ochrobactrum anthropi.Appl. Environ. Microbiol. 67 : 1783-1787 (2001). [PMID: 11282633]
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Enzyme-substrate interactions in the purine-specific nucleoside hydrolase from Trypanosoma vivax.J. Biol. Chem. 277 : 15938-15946 (2002). [PMID: 11854281]
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Computational study of IAG-nucleoside hydrolase: determination of the preferred ground state conformation and the role of active site residues.Biochemistry 44 : 7805-7817 (2005). [PMID: 15909995]
[EC 3.2.2.1 created 1961, modified 2006, modified 2011]