EC 3.2.1.67 - Galacturonan 1,4-α-galacturonidase

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IntEnz Enzyme Nomenclature
EC 3.2.1.67

Names

Accepted name:
galacturonan 1,4-α-galacturonidase
Other names:
exo-D-galacturonanase
exo-D-galacturonase
exopoly-D-galacturonase
exopolygalacturonase
poly(galacturonate) hydrolase [ambiguous]
poly(1,4-α-D-galacturonide) galacturonohydrolase [ambiguous]
pgaA (gene name)
galacturan 1,4-α-galacturonidase [incorrect]
Systematic name:
poly[(1→4)-α-D-galacturonide] non-reducing-end galacturonohydrolase

Reaction

Comments:

The enzyme hydrolyses the first glycosidic bond from the non-reducing end of the substrate. It is specific for saturated oligomers of D-homogalacturonan, and is unable to degrade unsaturated substrates or methyl-esterified substrates.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00415
Structural data: CSA , EC2PDB
Gene Ontology: GO:0047911
CAS Registry Number: 9045-35-6
UniProtKB/Swiss-Prot: (52) [show] [UniProt]

References

  1. Hasegawa, H. and Nagel, C.W.
    Isolation of an oligogalacturonate hydrolase from a Bacillus species.
    Arch. Biochem. Biophys. 124 : 513-520 (1968). [PMID: 5661621]
  2. Kluskens, L. D., van Alebeek, G. J., Walther, J., Voragen, A. G., de Vos, W. M., van der Oost, J.
    Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima.
    FEBS J. 272 : 5464-5473 (2005). [PMID: 16262687]
  3. Martens-Uzunova, E. S., Zandleven, J. S., Benen, J. A., Awad, H., Kools, H. J., Beldman, G., Voragen, A. G., Van den Berg, J. A., Schaap, P. J.
    A new group of exo-acting family 28 glycoside hydrolases of Aspergillus niger that are involved in pectin degradation.
    Biochem. J. 400 : 43-52 (2006). [PMID: 16822232]
  4. Pijning, T., van Pouderoyen, G., Kluskens, L., van der Oost, J., Dijkstra, B. W.
    The crystal structure of a hyperthermoactive exopolygalacturonase from Thermotoga maritima reveals a unique tetramer.
    FEBS Lett. 583 : 3665-3670 (2009). [PMID: 19854184]

[EC 3.2.1.67 created 1972, modified 2019]